製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ADH5
  • アプリケーション
    適用あり: ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide selected from the center region of Human ADH5, conjugated to KLH (NP_000662.3).

  • ポジティブ・コントロール
    • K562 cell lysate IF/ICC: HeLa cell line

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab91385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
WB
ELISA
  • 追加情報
    ELISA: 1/1000.
    WB: 1/100 - 1/500. Predicted molecular weight: 40 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione.
    • 配列類似性
      Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.
    • 細胞内局在
      Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ADH 3 antibody
      • ADH5 antibody
      • ADHX antibody
      • ADHX_HUMAN antibody
      • Alcohol dehydrogenase (class III) chi polypeptide antibody
      • alcohol dehydrogenase 5 (class III) chi polypeptide antibody
      • Alcohol dehydrogenase 5 antibody
      • Alcohol dehydrogenase class 3 antibody
      • Alcohol dehydrogenase class chi chain antibody
      • Alcohol dehydrogenase class III antibody
      • Alcohol dehydrogenase class-3 antibody
      • Alcohol dehydrogenase class-III antibody
      • class III alcohol dehydrogenase 5 chi subunit antibody
      • FALDH antibody
      • FDH antibody
      • formaldehyde dehydrogenase antibody
      • Glutathione dependent formaldehyde dehydrogenase antibody
      • Glutathione-dependent formaldehyde dehydrogenase antibody
      • GSH-FDH antibody
      • GSNOR antibody
      • hydroxymethyllutathione dehydrogenase antibody
      • S-(hydroxymethyl)glutathione dehydrogenase antibody
      see all

    Anti-ADH5 antibody 画像

    • ICC/IF image of ab91385 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91385, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

    • Anti-ADH5 antibody (ab91385) at 1/100 dilution + K562 cell lysate at 35 µg

      Predicted band size : 40 kDa

    Anti-ADH5 antibody (ab91385) 使用論文

    This product has been referenced in:
    • Zaman K  et al. Augmentation of CFTR maturation by S-nitrosoglutathione reductase. Am J Physiol Lung Cell Mol Physiol 310:L263-70 (2016). ICC/IF . Read more (PubMed: 26637637) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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