製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ADH1B
  • 特異性
    This antibody reacts with ADH1B
  • アプリケーション
    適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee
  • 免疫原

    A region within synthetic peptide: TSVIVGVPPA SQNLSINPML LLTGRTWKGA VYGGFKSKEG IPKLVADFMA, corresponding to C terminal amino acids 289-338 of Human ADH1B

  • ポジティブ・コントロール
    • Jurkat cell lysate and human kidney tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab58647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:1562500.

IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 配列類似性
    Belongs to the zinc-containing alcohol dehydrogenase family.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ADH beta subunit antibody
    • ADH1B antibody
    • ADH1B_HUMAN antibody
    • ADH2 antibody
    • Alcohol dehydrogenase 1B antibody
    • alcohol dehydrogenase 2 (class I), beta polypeptide antibody
    • Alcohol dehydrogenase 2 antibody
    • Alcohol dehydrogenase subunit beta antibody
    • Aldehyde reductase antibody
    • DKFZp686C06125 antibody
    • OTTHUMP00000220192 antibody
    see all

画像

  • Anti-ADH1B antibody (ab58647) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 40 kDa
    Observed band size : 40 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling ADH1B with ab58647 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubules. Magnification: 400X.

参考文献

ab58647 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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