製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ADFP
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human, Rhesus monkey
    交差が予測される動物種: Rat
  • 免疫原

    A synthetic peptide made to a C-terminal region within residues 350-425 of the mouse ADFP protein.

  • ポジティブ・コントロール
    • Mouse and human liver lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 20335253
WB Use a concentration of 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P 1/200.

ターゲット情報

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  • Immunohistochemical analysis of mouse liver tissue staining ADFP using ab52356. 

  • Anti-ADFP antibody (ab52356) at 2 µg/ml + mouse liver lysate

    Predicted band size: 48 kDa
    Observed band size: 48 kDa

  • Ab52356 staining ADFP in HeLa cells at 1/50 dilution. Hoechst 33258 was used as a nuclear counterstain. 

参考文献

This product has been referenced in:
  • Soayfane Z  et al. Exposure to dietary lipid leads to rapid production of cytosolic lipid droplets near the brush border membrane. Nutr Metab (Lond) 13:48 (2016). Read more (PubMed: 27478484) »
  • Ntokou A  et al. Characterization of the platelet-derived growth factor receptor-a-positive cell lineage during murine late lung development. Am J Physiol Lung Cell Mol Physiol 309:L942-58 (2015). Read more (PubMed: 26320158) »

See all 8 Publications for this product

レビューと Q&A

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse muscle)
Loading amount
40 µg
Specification
Mouse muscle
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Mar 25 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (AML-12 cell(liver cell line))
Loading amount
30 µg
Specification
AML-12 cell(liver cell line)
Treatment
2uM Rosiglitazone for 24hrs
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Oct 30 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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