製品の概要

  • 製品名Anti-Adenylate cyclase 1 antibody
    Adenylate cyclase 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Adenylate cyclase 1
  • 特異性Detects endogenous levels of total Adenylate cyclase 1 protein.
  • アプリケーション適用あり: IHC-P, WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide (Human) derived from an internal region of human Adenylate cyclase 1.

  • ポジティブ・コントロール
    • Extracts from COLO205 cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab69597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 124 kDa).
ELISA 1/40000.

ターゲット情報

  • 機能This is a calmodulin-sensitive adenylyl cyclase. May be involved in regulatory processes in the central nervous system. It may play a role in memory acquisition and learning.
  • 組織特異性Brain, retina and adrenal medulla.
  • 配列類似性Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
    Contains 2 guanylate cyclase domains.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Adcy1 antibody
    • ADCY1_HUMAN antibody
    • adenyl cyclase antibody
    • Adenylate cyclase 1 antibody
    • Adenylate cyclase type 1 antibody
    • Adenylate cyclase type I antibody
    • Adenylyl cyclase 1 antibody
    • ATP pyrophosphate lyase antibody
    • ATP pyrophosphate-lyase 1 antibody
    • Brain adenylate cyclase 1 antibody
    • Ca(2+)/calmodulin-activated adenylyl cyclase antibody
    see all

Anti-Adenylate cyclase 1 antibody 画像

  • All lanes : Anti-Adenylate cyclase 1 antibody (ab69597) at 1/500 dilution

    Lane 1 : Extracts from COLO205
    Lane 2 : Extracts from COLO205 with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 124 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 105 kDa,150 kDa,85 kDa. We are unsure as to the identity of these extra bands.
  • IHC image of ab69597 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab69597, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Adenylate cyclase 1 antibody (ab69597) 使用論文

This product has been referenced in:
  • Tekmen M & Gleason E Multiple Ca2+-dependent mechanisms regulate L-type Ca2+ current in retinal amacrine cells. J Neurophysiol 104:1849-66 (2010). WB ; Chicken . Read more (PubMed: 20685929) »

See 1 Publication for this product

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

To answer your question regarding the immunogen preparation and carrier, I have contacted our laborato...

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We do offer a number of products against the 10 Adenylate cyclase types however we do not currently have any products which will recognize all forms of this protein.

I have linked both a Unipro...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"