製品の概要

  • 製品名
    Anti-Adenovirus Type 5 E1A antibody [M58]
    Adenovirus Type 5 E1A 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [M58] to Adenovirus Type 5 E1A
  • 特異性
    ab31686 recognises Adenovirus, Type 5. The clone number has been updated from (0.N.8) to (M58) both clone numbers name the same antibody clone.
  • アプリケーション
    適用あり: IHC-P, IHC-FoFr, ICC/IF, IP, WB, IHC-Frmore details
  • 種交差性
    Adenovirus.
  • 免疫原

    Tissue/ cell preparation Adenovirus.

  • ポジティブ・コントロール
    • 293 cells or adenovirus infected cells and tissues.
  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 15mM Sodium Azide
    Constituents: 0.2% BSA, 10mM PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    M58
  • アイソタイプ
    IgG2a
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab31686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Incubate for 2 hours at room temperature.
IHC-FoFr Use a concentration of 2 - 4 µg/ml. Perform antigen retrieval before commencing the IHC protocol. Incubate for 2 hours at room temperature.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 35-46 kDa. Incubate for 2 hours at room temperature.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    The early region (E1A) of the adenovirus genome plays a central role in cellular transformation and regulation of gene expression. The E1A region encodes a series of related proteins (35-46kDa) with multifuctional capabilities and form a specific complex with the retinoblastoma tumor suppressor gene product (Rb protein). The E1A and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region. Several conserved regions of E1A are similar to portions of other viral oncoproteins like the HPV-16 and HPV-18 E7 and SV40 large T antigen.
  • 別名
    • Hadv 5 E1A antibody
    • HAdv5 E1A antibody

Anti-Adenovirus Type 5 E1A antibody [M58] (ab31686) 使用論文

This product has been referenced in:
  • Saha B & Parks RJ Human adenovirus type 5 vectors deleted of early region 1 (E1) undergo limited expression of early replicative E2 proteins and DNA replication in non-permissive cells. PLoS One 12:e0181012 (2017). WB ; Human . Read more (PubMed: 28700677) »
  • Turowski G  et al. Viral infection in placenta relevant cells - a morphological and immunohistochemical cell culture study. APMIS N/A:N/A (2014). Read more (PubMed: 25244625) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK 293)
Permeabilization
Yes - 0.1% Triton X100
Specification
HEK 293
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Apr 03 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Syrian Hamster Tissue sections (Brain. Infected NSC-GFP with adenovirus were injec)
Specification
Brain. Infected NSC-GFP with adenovirus were injec
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Bart Thaci

Verified customer

投稿 May 12 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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