製品の概要

  • 製品名Anti-Adenovirus Type 5 E1A antibody [0.N.8]
    Adenovirus Type 5 E1A 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [0.N.8] to Adenovirus Type 5 E1A
  • 特異性ab31686 recognises Adenovirus, Type 5.
  • アプリケーション適用あり: IHC-P, IHC-FoFr, ICC/IF, IP, WB, IHC-Frmore details
  • 種交差性
    Adenovirus.
  • 免疫原

    Tissue/ cell preparation Adenovirus.

  • ポジティブ・コントロール
    • 293 cells or adenovirus infected cells and tissues.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: 0.2% BSA, 10mM PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • クローン名0.N.8
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab31686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Incubate for 2 hours at room temperature.
IHC-FoFr Use a concentration of 2 - 4 µg/ml. Perform antigen retrieval before commencing the IHC protocol. Incubate for 2 hours at room temperature.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 35-46 kDa. Incubate for 2 hours at room temperature.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 関連性The early region (E1A) of the adenovirus genome plays a central role in cellular transformation and regulation of gene expression. The E1A region encodes a series of related proteins (35-46kDa) with multifuctional capabilities and form a specific complex with the retinoblastoma tumor suppressor gene product (Rb protein). The E1A and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region. Several conserved regions of E1A are similar to portions of other viral oncoproteins like the HPV-16 and HPV-18 E7 and SV40 large T antigen.
  • 別名
    • Hadv 5 E1A antibody
    • HAdv5 E1A antibody

Anti-Adenovirus Type 5 E1A antibody [0.N.8] (ab31686) 使用論文

This product has been referenced in:
  • Turowski G  et al. Viral infection in placenta relevant cells - a morphological and immunohistochemical cell culture study. APMIS N/A:N/A (2014). Read more (PubMed: 25244625) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Syrian Hamster Tissue sections (Brain. Infected NSC-GFP with adenovirus were injec)
Specification Brain. Infected NSC-GFP with adenovirus were injec
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Bart Thaci

Verified customer

投稿 May 12 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"