Anti-Adenovirus 抗体 [M58 + M73] (ab76550)

製品の概要

  • 製品名
    Anti-Adenovirus antibody [M58 + M73]
    Adenovirus 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [M58 + M73] to Adenovirus
  • アプリケーション
    適用あり: WB, IP, ICC/IF, IHC-Pmore details
  • 種交差性
    Adenovirus
  • 免疫原

    Adenovirus

  • ポジティブ・コントロール
    • 293 cells or adenovirus infected tissues
  • 特記事項

    This antibody detects adenovirus serogroups 2 and 5.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab7423 as a replacement.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 10mM PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein A purified
  • 一次抗体 備考
    This antibody detects adenovirus serogroups 2 and 5.
  • ポリ/モノ
    モノクローナル
  • クローン名
    M58 + M73
  • アイソタイプ
    IgG2a
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab76550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa.
IP Use at 2 µg/mg of lysate.

Native verified. Use Protein A.

ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

ターゲット情報

  • 関連性
    Adenoviruses are DNA viruses generally widespread in nature that are frequently the cause of acute upper respiratory tract infections (i.e. common colds). Forty-seven known serotypes have been isolated since they were first discovered in 1953 with 3 types known to cause gastroenteritis. Several types have oncogenic potential though most cause self-limiting febrile illnesses characterised by inflammation of conjunctivae and the respiratory tract. The virus can be isolated from the majority of tonsils/adenoids surgically removed, indicating latent infections. It is not known how long the virus can persist in the body, or whether it is capable of reactivation after long periods. In patients experiencing immunosuppression (e.g. AIDS) it can be reactivated causing disease.
  • 別名
    • Adenovirus antibody

画像

  • Formalin-fixed, paraffin-embedded human infected appendix stained with ab76550 using peroxidase-conjugate and DAB chromogen. Note nuclear and cytoplasmic staining of infected epithelial cells. This image was generated using a version of this antibody containing BSA and sodium azide.
  • Anti-Adenovirus antibody [M58 + M73] (ab76550) at 2 µg/ml + Human cells 1

    Observed band size : 41 kDa (why is the actual band size different from the predicted?)

参考文献

ab76550 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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