Anti-Adenosyl Homocysteine 抗体 [EPR4499] (ab111903)


  • 製品名Anti-Adenosyl Homocysteine antibody [EPR4499]
  • 製品の詳細
    Rabbit monoclonal [EPR4499] to Adenosyl Homocysteine
  • 特異性ab111903 shows the following reactivities with related compounds: S-(5'-Adenosyl)-L-homocysteine: 100% S-(5'-Adenosyl)-L-methionine: 0% Adenosine: 4.4% Homocysteine: 4.4% Cystathionine: 0% L-Cysteine: 1.5% Glutathione: 1.8%
  • アプリケーション適用あり: Competitive ELISAmore details
  • 免疫原

    Adenosyl Homocysteine conjugated to KLH.

  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab111903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Competitive ELISA 1/5000.


  • 関連性Adenosyl Homocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependant methyl transferase reactions and therefore may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.
  • 細胞内局在Cytoplasmic
  • 別名
    • S (5' Adenosyl) L homocysteine antibody
    • S Adenosyl L homocysteine antibody
    • S adenosylhomocysteine antibody
    • SAH antibody

Anti-Adenosyl Homocysteine antibody [EPR4499] 画像

  • Competitive ELISA: 0.1 µg/ml of BSA-S-adenosylhomocysteine was coated into 96 wells. Serial dilution of S-adenosylhomocysteine (SAH), SAMe, et al. and 0.05 µg/ml of ab111903 were added. HRP conjugated Goat anti-Rabbit IgG antibody was used to develop the color.

Anti-Adenosyl Homocysteine antibody [EPR4499] (ab111903) 使用論文

This product has been referenced in:
  • Wu C & Tzertzinis G Selection of a mimotope peptide of S-adenosyl-L-homocysteine and its application in immunoassays. Molecules 18:13020-6 (2013). ELISA . Read more (PubMed: 24145794) »

See 1 Publication for this product

Product Wall

The competitive ELISA protocol is as follow:

1. 0.1 ug/ml of BSA-S-adenosylhomocysteine was coated into 96 wells.50ul/well,4 ℃,overnight.

2. Add 120µl/well of 1% BSA-TBS and incubate for 1 hours at RT.

3.Wash the plate...

Read More

Thank you for your enquiry.

I can confirm that ab111903 antibody is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated ...

Read More

I meant to add that if you would be able to explain a little more fully what it is you are studying and what you are trying to achieve, I may be able to help you with other ideas of how to achieve it.
I look forward to your reply.

I have had a chat to my colleague who is very experienced with flow cytometry in regards to how to optimise these antibodies for this application.
Unfortunately, neither of these antibodies have been used for this application previously and optimi...

Read More

Thank you for your enquiry.

I can confirm that Anti-Adenosyl Homocysteine antibody (ab111903) is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will n...

Read More

Thank you for providing that extra information. It is unfortunate that the ab111903 has not out-performed ab35164 so far.

Do you see any signal at all with ab111903? The only thing I can suggest having looked at your protocol and taking into...

Read More

Sorry for the delay in getting back to you.

I am very sorry to hear that the ab111903 has not performed as well as ab35164. In order to try and understand what is going on would you please be able to send me some protocol details again....

Read More

I'm glad you have seen improvements in using ab35164. May I ask, what do you think has lead to these improvements? It would be very useful information to know in order to advise customers in the future.

Unfortunately due to the size of ...

Read More

Thank you for your enquiry.

You are correct that there is moderate cross-reactivity to SAMe. I'd put this in the range of 1-4%. I apologize for the error and will have the datasheet updated.

I hope this is helpful. Please contact me...

Read More

Thank you for the extra details you provided. In answer to your questions:

1. Could you possibly tell which supplier of SAH you used for standards when testing your antibody, as we have discovered that it may be susceptible to oxidation in bo...

Read More