Anti-Adenosine Receptor A2a 抗体 [7F6-G5-A2] (ab79714)

製品の概要

  • 製品名Anti-Adenosine Receptor A2a antibody [7F6-G5-A2]
    Adenosine Receptor A2a 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [7F6-G5-A2] to Adenosine Receptor A2a
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Guinea pig, Hamster, Dog, Non Human Primates
  • 免疫原

    Full length protein (Human)

  • エピトープRecognises amino acids 213-220 (SQPLPGER) within the third intracellular loop.
  • ポジティブ・コントロール
    • This antibody gave a positive result when used in the following methanol fixed cell lines: SKNSH In western blot, this antibody gave a positive result in SHSY-5Y, SK-N-SH, PC12 and human platelet whole cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab79714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 45 kDa).

We recommend using 1% BSA as a blocking agent for western blot.

ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
  • 配列類似性Belongs to the G-protein coupled receptor 1 family.
  • ドメインThe cytoplasmic C-terminal domain is necessary for targeting the non-ubiquitinated form of this protein to the cell surface.
  • 翻訳後修飾Ubiquitinated. Deubiquitinated by USP4; leading to stabilization and expression at the cell surface.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A2AAR antibody
    • A2aR antibody
    • AA2AR_HUMAN antibody
    • ADENO antibody
    • Adenosine A2 receptor antibody
    • Adenosine A2a receptor antibody
    • Adenosine receptor A2a antibody
    • Adenosine receptor subtype A2a antibody
    • ADORA 2 antibody
    • ADORA2 antibody
    • ADORA2A antibody
    • hA2aR antibody
    • RDC 8 antibody
    • RDC8 antibody
    see all

Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] 画像

  • All lanes : Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714) at 5 µg/ml

    Lane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 2 : SK-N-SH (Human neuroblastoma) Whole Cell Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 4 : Platelet (Human) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa


    Exposure time : 2 minutes

    We recommend using 1% BSA as a blocking agent for western blot. Milk is not a suitable blocking agent for this product.

  • ICC/IF image of ab79714 stained SKNSH cells. The cells were 100% methanol fixed (5 min) then permeabilised using 0.1% PBS-Triton and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to further permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab79714 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- mouse (ab150117) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (ab79714) 使用論文

This product has been referenced in:
  • Niesman IR  et al. Traumatic brain injury enhances neuroinflammation and lesion volume in caveolin deficient mice. J Neuroinflammation 11:39 (2014). WB ; Mouse . Read more (PubMed: 24593993) »
  • Bone DB  et al. Nucleoside/nucleobase transport and metabolism by microvascular endothelial cells isolated from ENT1-/- mice. Am J Physiol Heart Circ Physiol 299:H847-56 (2010). WB ; Mouse . Read more (PubMed: 20543083) »

See all 2 Publications for this product

Product Wall

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (8)
Sample Human Tissue lysate - whole (Heart)
Specification Heart
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jun 10 2013

Thank you for that reply.

Kate is currently away from the office but I will try to help as best I can with your enquiry. I have checked the two secondary antibodies intended to be used and they seem like they should perform fine with the prima...

Read More

Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments.

Before proce...

Read More

Thank you for your message and for kindly confirming these details.

I look forward to hearing from you with the results from the samples lysed in RIPA. Please do not hesitate to let me know how you get on. If this still does not work with RI...

Read More

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this...

Read More

Thank you for your reply.

I can confirm that I send you the quote for ab79714 in a separate email.

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guar...

Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HepG2)
Specification HepG2
Fixative Paraformaldehyde
Permeabilization Yes - PBS containing 0.2% Triton X100
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
Username

Abcam user community

Verified customer

投稿 Mar 18 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"