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Our Abpromise guarantee covers the use of ab40002 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 37 kDa).|
|IHC-P||Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
ab40002 (2µg/ml) staining of paraffin embedded Human Kidney shows cytoplasm staining in cells of renal tubules following steamed antigen retrieval with citrate buffer pH 6 and HRP-staining.
This image is courtesy of an Abreview submitted by Dr Prit Gill
ab40002 at 3ug/ml staining Adenosine A2b receptor in human kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in microwave with Tris/EDTA buffer (pH 9.0). The HRP staining procedure was used for detection.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"