Anti-Adenomatous polyposis coli protein 2 抗体 (ab80018)

製品の概要

  • 製品名
    Anti-Adenomatous polyposis coli protein 2 antibody
    Adenomatous polyposis coli protein 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Adenomatous polyposis coli protein 2
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human Adenomatous polyposis coli protein 2 aa 2250 to the C-terminus conjugated to keyhole limpet haemocyanin.
    Database link: O95996
    (Peptide available as ab88193)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in human spinal cord tissue lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab80018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution. Detects a band of approximately 213 kDa (predicted molecular weight: 243 kDa).

ターゲット情報

  • 機能
    Promotes rapid degradation of CTNNB1 and may function as a tumor suppressor. May function in Wnt signaling.
  • 組織特異性
    Widely expressed (at protein level). Specifically expressed in the CNS.
  • 配列類似性
    Belongs to the adenomatous polyposis coli (APC) family.
    Contains 6 ARM repeats.
  • 発生段階
    Expressed in fetal brain.
  • 細胞内局在
    Cytoplasm, cytoskeleton. Golgi apparatus. Cytoplasm. Cytoplasm, perinuclear region. Associated with actin filaments (PubMed:11691822). Associated with microtubule network (PubMed:10644998, PubMed:11691822).
  • Information by UniProt
  • 参照データベース
  • 別名
    • Adenomatous polyposis coli protein 2 antibody
    • Adenomatous polyposis coli protein-like antibody
    • APC-like antibody
    • apc2 antibody
    • APC2_HUMAN antibody
    • APCL antibody
    see all

Anti-Adenomatous polyposis coli protein 2 antibody 画像

  • Anti-Adenomatous polyposis coli protein 2 antibody (ab80018) at 1 µg/ml + Human spinal cord tissue lysate - total protein (ab29188) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 243 kDa
    Observed band size : 213 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 secondsab80018 detects a strong band at 213 kDa in human spinal cord tissue lysate. This antibody was raised against an immunogen that is predicted to cross react with all known isoforms of adenomatous polyposis coli protein 2 (APC2). APC2 has three isoforms, isoform one has a molecular weight of 243 kDa with isoforms two and three a molecular weight of 213 and 243 respectively(SwissProt data). The band we have observed is thought to represent isoform 2 of APC2 (213 kDa).

プロトコール

Anti-Adenomatous polyposis coli protein 2 antibody (ab80018) 使用論文

ab80018 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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