製品の概要

  • 製品名Anti-ADCY10 antibody
    ADCY10 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ADCY10
  • アプリケーション適用あり: IHC-Fr, WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 396-445 (VGHTVRHEYT VIGQKVNLAA RMMMYYPGIV TCDSVTYNGS NLPAYFFKEL) of human ADCY10 (NP_060887).

  • ポジティブ・コントロール
    • Human fetal brain lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab82854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration. PubMed: 22649251
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 187 kDa.Can be blocked with ADCY10 peptide (ab211578). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 関連性ADCY10 belongs to a distinct class of mammalian adenylyl cyclase that is soluble and insensitive to G protein or forskolin regulation. It is localized in the cytoplasm and is thought to function as a general bicarbonate sensor throughout the body. It may also play an important role in the generation of cAMP in spermatozoa, implying possible roles in sperm maturation through the epididymis, capacitation, hypermotility, and/or the acrosome reaction.
  • 細胞内局在Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region. Note: Distributed to subcellular compartments containing cAMP targets. Found as a plasma membrane associated protein, protein concentrated in the perinuclear region and protein colocalized with actin or tubulin.
  • 参照データベース
  • 別名
    • 3',5'-cyclic AMP synthetase antibody
    • Adenylate cyclase 10 (soluble) antibody
    • Adenylate cyclase 10 antibody
    • Adenylate cyclase homolog antibody
    • AH related protein antibody
    • ATP pyrophosphate-lyase antibody
    • Germ cell soluble adenylyl cyclase antibody
    • HCA2 antibody
    • hsAC antibody
    • hypercalciuria absorptive antibody
    • SAC antibody
    • SACI antibody
    • Sacy antibody
    • Soluble adenylyl cyclase antibody
    • testicular soluble adenylyl cyclase (SAC) antibody
    • Testicular soluble adenylyl cyclase antibody
    see all

Anti-ADCY10 antibody 画像

  • Anti-ADCY10 antibody (ab82854) at 1 µg/ml (in 5% skim milk / PBS buffer) + Fetal Brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 187 kDa
    Observed band size : 145 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

Anti-ADCY10 antibody (ab82854) 使用論文

This product has been referenced in:
  • Corredor RG  et al. Soluble adenylyl cyclase activity is necessary for retinal ganglion cell survival and axon growth. J Neurosci 32:7734-44 (2012). IHC-Fr ; Rat . Read more (PubMed: 22649251) »

See 1 Publication for this product

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1.) I suggest to use 10-30ug of total protein per well on the gel. This might have to be optimised, but I recommend to start with 20ug and optimise form the...

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