製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ADAT1
  • アプリケーション
    適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide: AISWSAVPEQ PLDVTANGFP QGTTKKTIGS LQARSQISKV ELFRSFQKLL, corresponding to amino acids 397-446 of Human ADAT1

  • ポジティブ・コントロール
    • Jurkat cell lysate and Human heart tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

ターゲット情報

画像

  • Lane 1 : MW marker
    Lane 2 : Anti-ADAT1 antibody (ab50994) at 1.25 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 39 kDa
    Observed band size : 39 kDa
  • ab50994 staining of Paraffin embeded Human heart tissue in IHC application at 4-8 ug/ml dilution. Cells with positive label (Myocardial cells) are indicated with arrows. Magnification: 400x

参考文献

ab50994 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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