製品の概要

  • 製品名Anti-ADAMTSL3 antibody
  • 製品の詳細
    Rabbit polyclonal to ADAMTSL3
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Recombinant fragment, corresponding to amino acids 529-788 of Human ADAMTSL3 (BC128389).

  • ポジティブ・コントロール
    • Human Fetal Kidney

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab104507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2000. Predicted molecular weight: 189 kDa.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 組織特異性Expressed in epithelial cells of the colon, fallopian tube, skin, breast, prostate, epididymis, liver, pancreatic islets and bile ducts, as well as by vascular endothelial cells, smooth muscle cells, fibroblasts, cortical and ganglionic neurons and cardiac myocytes. Also expressed by malignant epithelial cells in colon cancer, as well as breast, prostate, renal and skin tumors. Expression is significantly reduced in colon cancer compared to normal colon.
  • 配列類似性Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 PLAC domain.
    Contains 10 TSP type-1 domains.
  • 翻訳後修飾Glycosylated (By similarity). Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion.
  • 細胞内局在Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A disintegrin like and metalloprotease domain with thrombospondin type I motifs like 3 antibody
    • ADAMTS like 3 antibody
    • ADAMTS-like protein 3 antibody
    • ADAMTSL-3 antibody
    • ADAMTSL3 antibody
    • ATL3_HUMAN antibody
    • Punctin-2 antibody
    see all

Anti-ADAMTSL3 antibody 画像

  • ab104507 staining ADAMTSL3 in Mouse heart valve cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 0.02% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/25) for 16 hour at 4°C. An Alexa Fluor®594-conjugated Goat anti-rabbit IgG polyclonal(1/200) was used as the secondary antibody.

    See Abreview

  • Anti-ADAMTSL3 antibody (ab104507) at 1/1000 dilution + Human Fetal Kidney Lysate

    Predicted band size : 189 kDa

Anti-ADAMTSL3 antibody (ab104507) 使用論文

ab104507 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Heart Valve)
Specification Heart Valve
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: .02% · Temperature: 25°C
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投稿 Apr 10 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"