製品の概要

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab45048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: 1/1000 - 1/5000. Predicted molecular weight: 136 kDa. The recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性ADAMTS16 is a member of the ADAMs family of proteinases with 4 thrombospondin-like domains. ADAMTS16 is closest in homology to ADAMTS18, sharing 57% overall identity. ADAMTS16 is expressed in fetal human lung and kidney, and in adult brain and ovary as well as in osteoarthritic cartilage, prostate and breast tissue. ADAMTS16 contains a prohormone-convertase cleavage site; the predicted PC cleavage site is the RRRR87 sequence in the 1224 and 1072 amino acid forms. This catalytic site is typical of the metalloproteinase catalytic domains, with an HExxHxxxxxH sequence. Several splice variants of ADAMTS16 have been reported.
    • 細胞内局在Secreted
    • 参照データベース
    • 別名
      • A disintegrin and metalloproteinase with thrombospondin motifs 16 antibody
      • ADAMTS 16 antibody
      • ADAMTS16 antibody
      • EC 3.4.24. antibody

    Anti-ADAMTS16 antibody 画像

    • All lanes : Anti-ADAMTS16 antibody (ab45048) at 1/1000 dilution

      Lane 1 : Whole cell lysate prepared from mouse lung mesenchymal MLg cells (treated with SF)
      Lane 2 : Whole cell lysate prepared from mouse lung mesenchymal MLg cells (treated with TPA)
      Lane 3 : Whole cell lysate prepared from mouse lung mesenchymal MLg cells (treated with IL1-alpha)
      Lane 4 : Whole cell lysate prepared from mouse lung mesenchymal MLg cells (treated with RA)


      Predicted band size : 136 kDa

    プロトコール

    Anti-ADAMTS16 antibody (ab45048) 使用論文

    This product has been referenced in:
    • Jacobi CL  et al. Transcriptional regulation by the Wilms tumor protein, Wt1, suggests a role of the metalloproteinase Adamts16 in murine genitourinary development. J Biol Chem 288:18811-24 (2013). Read more (PubMed: 23661704) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"