製品の概要

  • 製品名Anti-ADAMTS15 antibody
    ADAMTS15 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ADAMTS15
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide based on the propeptide region of human ADAMTS-15 ADAMTS15.(Peptide available as ab45243.)

  • 特記事項We have a range of domain specific antibodies for this target. For a full list please see all ADAMTS15 antibodies.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab45047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: 1/1000 - 1/5000. Predicted molecular weight: 103 kDa. The recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 組織特異性Expressed in fetal liver and kidney, but not in any of the adult tissues examined.
    • 配列類似性Contains 1 disintegrin domain.
      Contains 1 peptidase M12B domain.
      Contains 3 TSP type-1 domains.
    • ドメインThe spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • 翻訳後修飾The precursor is cleaved by a furin endopeptidase.
      Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion.
    • 細胞内局在Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • 参照データベース
    • 別名
      • A disintegrin and metalloproteinase with thrombospondin motifs 15 antibody
      • A disintegrin like and metalloprotease reprolysin type with thrombospondin type 1 motif 15 antibody
      • A disintegrin like and metalloprotease reprolysin type with thrombospondin type 1 motif 15 preproprotein antibody
      • A disintegrin like and metalloprotease with thrombospondin type 1 motif 15 antibody
      • A disintegrin like and metalloprotease with thrombospondin type 1 motif 15 preproprotein antibody
      • ADAM metallopeptidase with thrombospondin type 1 motif 15 antibody
      • ADAM TS 15 antibody
      • ADAM TS15 antibody
      • ADAM-TS 15 antibody
      • ADAM-TS15 antibody
      • ADAMTS 15 antibody
      • ADAMTS-15 antibody
      • ADAMTS15 antibody
      • ATS15_HUMAN antibody
      • MGC126403 antibody
      see all

    プロトコール

    Anti-ADAMTS15 antibody (ab45047) 使用論文

    This product has been referenced in:
    • Dancevic CM  et al. Biosynthesis and expression of a disintegrin-like and metalloproteinase domain with thrombospondin-1 repeats-15: a novel versican-cleaving proteoglycanase. J Biol Chem 288:37267-76 (2013). WB ; Human . Read more (PubMed: 24220035) »
    • Molokwu CN  et al. Androgen regulates ADAMTS15 gene expression in prostate cancer cells. Cancer Invest 28:698-710 (2010). WB ; Human . Read more (PubMed: 20590445) »

    See all 2 Publications for this product

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (MDAMB231 breast cancer cells)
    Loading amount 30 µg
    Specification MDAMB231 breast cancer cells
    Gel Running Conditions Non-reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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    投稿 Apr 05 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"