製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ADAMTS13
  • 特異性
    ab28274 recognises the metalloproteinase domain of ADAMTS13.
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human ADAMTS13.
    (Peptide available as ab41249)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000. Detects a band of approximately 190 kDa (predicted molecular weight: 154 kDa).Can be blocked with ADAMTS13 peptide (ab41249). 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000, when using chemiluminescent substrates. Glycosylation and the abundance of cysteine residues gives ADAMTS 13 an apparent molecular weight of 190 kDa on reduced SDS PAGE gels. Several bands at 110-190 kDa are observed on Western blots, possibly indicating differentially processed ADAMTS 13. Dilution optimised using Chromogenic detection.

ターゲット情報

  • 機能
    Cleaves the vWF multimers in plasma into smaller forms.
  • 組織特異性
    Plasma. Expressed primarily in liver.
  • 関連疾患
    Defects in ADAMTS13 are the cause of thrombotic thrombocytopenic purpura congenital (TTP) [MIM:274150]; also known as Upshaw-Schulman syndrome (USS). A hematologic disease characterized by hemolytic anemia with fragmentation of erythrocytes, thrombocytopenia, diffuse and non-focal neurologic findings, decreased renal function and fever.
  • 配列類似性
    Contains 2 CUB domains.
    Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
    Contains 8 TSP type-1 domains.
  • ドメイン
    The pro-domain is not required for folding or secretion and does not perform the common function of maintening enzyme latency.
    The spacer domain is necessary to recognize and cleave vWF. The C-terminal TSP type-1 and CUB domains may modulate this interaction.
  • 翻訳後修飾
    May contain a C-mannosylation site and O-fucosylation sites in the TSP type-1 domains.
    The precursor is processed by a furin endopeptidase which cleaves off the pro-domain.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A disintegrin and metalloproteinase with thrombospondin motifs 13 antibody
    • A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 13 antibody
    • A disintegrin like and metalloprotease with thrombospondin type 1 motif 13 antibody
    • ADAM metallopeptidase with thrombospondin type 1 motif 13 antibody
    • ADAM TS antibody
    • ADAM-TS 13 antibody
    • ADAM-TS13 antibody
    • ADAMTS 13 antibody
    • ADAMTS-13 antibody
    • ADAMTS13 antibody
    • ADAMTS13 protein antibody
    • ATS13_HUMAN antibody
    • C9orf8 antibody
    • TTP antibody
    • Von Willebrand factor cleaving protease antibody
    • von Willebrand factor-cleaving protease antibody
    • vWF cleaving protease antibody
    • vWF CP antibody
    • vWF-cleaving protease antibody
    • vWF-CP antibody
    • vWFCP antibody
    see all

画像

  • All lanes : Anti-ADAMTS13 antibody (ab28274) at 1 µg/ml

    Lane 1 : Recombinant Human ATS-13 at 0.08 µg
    Lane 2 : Recombinant Human ATS-13 at 0.04 µg
    Lane 3 : Recombinant Human ATS-13 at 0.02 µg


    Predicted band size : 154 kDa

プロトコール

参考文献

This product has been referenced in:

See 1 Publication for this product

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (hepatocytes)
Permeabilization
Yes - 0.1% Triton X-100
Specification
hepatocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative
Paraformaldehyde
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投稿 Dec 15 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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