製品の概要

  • 製品名Anti-ADAMTS13 antibody
    ADAMTS13 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ADAMTS13
  • 特異性ab28273 recognises the Carboxyterminal end of the long form of ADAMTS13.
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide based on the carboxyterminal end of full length human ADAMTS13. (Peptide available as ab41251.)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB 1/1000 - 1/5000. Detects a band of approximately 190 kDa (predicted molecular weight: 154 kDa). 1/1000, when using colorimetric substrates such as BCIP/NBT - 1/5000, when using chemiluminescent substrates. Detects a band of approximately 190 kDa. Glycosylation and the abundance of cysteine residues gives ADAMTS13 an apparent molecular weight of 190 kDa on reduced SDS PAGE gels. Several bands between 110-190 kDa are observed on Western blots, possibly indicating differentially processed ADAMTS 13. Dilution optimised using Chromogenic detection.

ターゲット情報

  • 機能Cleaves the vWF multimers in plasma into smaller forms.
  • 組織特異性Plasma. Expressed primarily in liver.
  • 関連疾患Defects in ADAMTS13 are the cause of thrombotic thrombocytopenic purpura congenital (TTP) [MIM:274150]; also known as Upshaw-Schulman syndrome (USS). A hematologic disease characterized by hemolytic anemia with fragmentation of erythrocytes, thrombocytopenia, diffuse and non-focal neurologic findings, decreased renal function and fever.
  • 配列類似性Contains 2 CUB domains.
    Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
    Contains 8 TSP type-1 domains.
  • ドメインThe pro-domain is not required for folding or secretion and does not perform the common function of maintening enzyme latency.
    The spacer domain is necessary to recognize and cleave vWF. The C-terminal TSP type-1 and CUB domains may modulate this interaction.
  • 翻訳後修飾May contain a C-mannosylation site and O-fucosylation sites in the TSP type-1 domains.
    The precursor is processed by a furin endopeptidase which cleaves off the pro-domain.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A disintegrin and metalloproteinase with thrombospondin motifs 13 antibody
    • A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 13 antibody
    • A disintegrin like and metalloprotease with thrombospondin type 1 motif 13 antibody
    • ADAM metallopeptidase with thrombospondin type 1 motif 13 antibody
    • ADAM TS antibody
    • ADAM-TS 13 antibody
    • ADAM-TS13 antibody
    • ADAMTS 13 antibody
    • ADAMTS-13 antibody
    • ADAMTS13 antibody
    • ADAMTS13 protein antibody
    • ATS13_HUMAN antibody
    • C9orf8 antibody
    • TTP antibody
    • Von Willebrand factor cleaving protease antibody
    • von Willebrand factor-cleaving protease antibody
    • vWF cleaving protease antibody
    • vWF CP antibody
    • vWF-cleaving protease antibody
    • vWF-CP antibody
    • vWFCP antibody
    see all

Anti-ADAMTS13 antibody 画像

  • All lanes : Anti-ADAMTS13 antibody (ab28273) at 1 µg/ml

    Lane 1 : Recombinant Human ATS-13 at 0.08 µg
    Lane 2 : Recombinant Human ATS-13 at 0.04 µg
    Lane 3 : Recombinant Human ATS-13 at 0.02 µg


    Predicted band size : 154 kDa
  • ICC/IF image of ab28273 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28273, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

プロトコール

Anti-ADAMTS13 antibody (ab28273) 使用論文

ab28273 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your patience in this matter. The originator of ab28273 (ADAMTS13 antibody - Carboxyterminal end long isoform) has informed us that the immunogenic peptide sequence used to raise this antibody lies within the last 35 amino acids of the...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"