製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab45041 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: 1/1000 - 1/5000. Predicted molecular weight: 178 kDa. The recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性This protein is a disintegrin and metalloproteinase with thrombospondin motifs 12 (ADAMTS12), which is a member of the ADAMTS protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin like domain, and a thrombospondin type 1 (TS 1) motif. Individual members of this family differ in the number of C terminal TS 1 motifs, and some have unique C terminal domains. The enzyme encoded by this gene contains 8 TS 1 motifs. It may play roles in pulmonary cells during fetal development or in tumor processes through its proteolytic activity or as a molecule potentially involved in regulation of cell adhesion.
    • 細胞内局在Secreted
    • 参照データベース
    • 別名
      • A disintegrin and metalloproteinase with thrombospondin motifs 12 antibody
      • A disintegrin like and metalloprotease with thrombospondin type 1 motif 12 antibody
      • ADAM metallopeptidase with thrombospondin type 1 motif 12 antibody
      • ADAM TS 12 antibody

    Anti-ADAMTS12 antibody 画像

    • All lanes : Anti-ADAMTS12 antibody (ab45041) at 1/1000 dilution

      Lane 1 : Cell media from mouse squamous cell carcinoma KLN 205 cells (treated with TPA)
      Lane 2 : Cell media from mouse squamous cell carcinoma KLN 205 cells (treated with IL1-alpha)
      Lane 3 : Cell media from mouse squamous cell carcinoma KLN 205 cells (treated with TNF-alpha)


      Predicted band size : 178 kDa

    プロトコール

    Anti-ADAMTS12 antibody (ab45041) 使用論文

    This product has been referenced in:
    • Acar-Perk B  et al. The t(14,15) in mouse strain CBA/CaH-T(14;15)6Ca/J causes a break in the ADAMTS12 gene. Comp Med 60:118-22 (2010). WB ; Mouse . Read more (PubMed: 20412686) »

    See 1 Publication for this product

    Product Wall

    Gracias por su respuesta. Desafortunadamente, no tenemos lisados de cartilago en nuestro catalogo. Nosotros tenemos un lisado de tumor en hueso, pero no estoy segura si eso contiene condrocitos, este producto es ab29359. Espero que esto sea de alguna a...

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    Gracias por contactarnos. Para ab45041, nosotros recomendamos cerebro (ab30069) como un control positivo. Desafortunadamente, ADAMTS7 solo es expresado en plaquetas y medula óssea y no vendemos esos lisados. Podria ser que encuentres algo usable...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"