製品の概要

  • 製品名Anti-ADAM17 antibody
    ADAM17 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to ADAM17
  • アプリケーション適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant fragment: RADPDPMKNT CKLLVVADHR FYRYMGRGEE STTTNYLIEL IDRVDDIYRN TSWDNAGFKG YGIQIEQIRI LKSPQEVKPG EKHYNMAKSY PNEEKDAWDV , corresponding to amino acids 215-315 of Human ADAM17

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab57484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1µg for 106 cells. (methanol fixed cells)



ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway.
  • 組織特異性Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.
  • 配列類似性Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
  • ドメインMust be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻訳後修飾The precursor is cleaved by a furin endopeptidase.
    Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) antibody
    • A disintegrin and metalloproteinase domain 17 antibody
    • ADA17_HUMAN antibody
    • ADAM 17 antibody
    • ADAM metallopeptidase domain 17 antibody
    • ADAM17 antibody
    • ADAM17 protein antibody
    • CD 156b antibody
    • CD156b antibody
    • CD156b antigen antibody
    • CSVP antibody
    • Disintegrin and metalloproteinase domain-containing protein 17 antibody
    • MGC71942 antibody
    • NISBD antibody
    • NISBD1 antibody
    • Snake venom like protease antibody
    • Snake venom-like protease antibody
    • TACE antibody
    • TNF alpha convertase antibody
    • TNF alpha converting enzyme antibody
    • TNF-alpha convertase antibody
    • TNF-alpha-converting enzyme antibody
    • Tumor Necrosis Factor Alpha Converting Enzyme antibody
    see all

Anti-ADAM17 antibody 画像

  • ADAM17 antibody (ab57484) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human small Intestine.
  • Western blot against tagged recombinant protein immunogen using ab57484 ADAM17 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
  • ab57484 was used to detect mouse ADAM17 overexpressed in Hela cells. The transfected cells were incubated with ab57484 (10 µg/ml ) for 1 hour at  22°C. For further details please refer to the Abreview.

  • Overlay histogram showing HeLa cells stained with ab57484 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57484, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

    Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • Anti-ADAM17 antibody (ab57484) at 1/1000 dilution + whole cell lysate prepared from MCF7 cells at 100000 cells

    Secondary
    HRP conjugated sheep anti-mouse polyclonal at 1/2000 dilution
    Developed using the ECL technique

    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    Image courtesy of an anonymous Abreview.

    See Abreview

Anti-ADAM17 antibody (ab57484) 使用論文

This product has been referenced in:
  • McClurg UL  et al. Epithelial cell ADAM17 activation by Helicobacter pylori: role of ADAM17 C-terminus and Threonine-735 phosphorylation. Microbes Infect 17:205-14 (2015). Read more (PubMed: 25499189) »
  • Liu Y  et al. Expression of miR-224, miR-145, and their putative target ADAM17 in hepatocellular carcinoma. Acta Biochim Biophys Sin (Shanghai) 46:720-2 (2014). Read more (PubMed: 24969373) »

See all 6 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (mcf7)
Loading amount 100000 cells
Specification mcf7
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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投稿 May 11 2012

Application Western blot
Sample Human Cell lysate - whole cell (Cell lines)
Loading amount 50 µg
Specification Cell lines
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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投稿 Feb 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HT1080 Cell line)
Loading amount 20000 cells
Specification HT1080 Cell line
Gel Running Conditions Reduced Denaturing (9%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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投稿 Jan 05 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (H357 (oral SCC))
Specification H357 (oral SCC)
Fixative Acetone/methanol
Permeabilization No
Blocking step BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
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投稿 Jul 03 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hela Cells transiently overexpressing mouse ADAM17)
Specification Hela Cells transiently overexpressing mouse ADAM17
Fixative Methanol
Permeabilization No
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投稿 Mar 05 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"