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Synthetic peptide based on the propeptide domain of human ADAM17.
(Peptide available as ab41213.)
Our Abpromise guarantee covers the use of ab39161 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 93 kDa). 1/1000 (when using colorimetric substrates such as BCIP/NBT) and 1/5000 (for chemiluminescent substrates). Higher concentrations of antibody may be needed for samples from more distantly related species. The antibody recognizes a 110 kDa band in reduced Western blots of conditioned media or cell lysates, which is rapidly converted to a 55-60 kDa band. Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection.|
|ICC/IF||Use a concentration of 10 µg/ml.|
Methanol fixed HeLa cells incubated with ab39161 at 10 µg/ml for 1 hour at 22°C. Clear membrane staining can be seen. For further details please refer to the Abreview.