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Our Abpromise guarantee covers the use of ab28233 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 120 kDa. ab28233 recognizes a 120 kDa band in reduced Western blots of conditioned media or cell lysates, which is converted to a 55-60 kDa band. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Note: 1/1,000 when using colorimetric substrates such as BCIP/NBT, and 1/5,000 for chemiluminescent substrates. Dilution optimised using Chromogenic detection.|
|ICC/IF||Use a concentration of 10 µg/ml.|
|IP||Use at an assay dependent concentration.|
Methanol fixed Hela cells stained with ab28233 (10 µg/ml) for 1 hour at 22°C. Clear staining can be seen. For further details please refer to the Abreview