Anti-ADAM15 抗体 [EPR5619] (ab124698)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5619] to ADAM15
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ADAM15 antibody [EPR5619]
ADAM15 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5619] to ADAM15 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human ADAM15 aa 250-350 (extracellular). The exact sequence is proprietary.
Database link: Q13444 -
ポジティブ・コントロール
- SW480 cell lysate; Human colon tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 5.86 x 10 -11 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5619 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124698の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 90, 110 kDa (predicted molecular weight: 93 kDa).
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IHC-P |
1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
For unpurified use 1/250.00000 - 1/500.00000. See IHC antigen retrieval protocols. |
特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 90, 110 kDa (predicted molecular weight: 93 kDa). |
IHC-P
1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. For unpurified use 1/250.00000 - 1/500.00000. See IHC antigen retrieval protocols. |
ターゲット情報
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機能
Active metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain. -
組織特異性
Expressed in colon and small intestine. Expressed in airway smooth muscle and glomerular mesangial cells (at protein level). Ubiquitously expressed. Overexpressed in atherosclerotic lesions. Constitutively expressed in cultured endothelium and smooth muscle. Expressed in chondrocytes. Expressed in airway smooth muscle and glomerular mesangial cells. -
配列類似性
Contains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain. -
ドメイン
The cytoplasmic domain interacts with endophilin I and sorting nexin 9.
Disintegrin domain binds to integrin alphaV-beta3.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻訳後修飾
The precursor is cleaved by a furin endopeptidase.
Phosphorylation increases association with PTKs. -
細胞内局在
Endomembrane system. Cell junction > adherens junction. Cell projection > cilium > flagellum. Cytoplasmic vesicle > secretory vesicle > acrosome. The majority of the protein is localized in a perinuclear compartment which may correspond to the trans-Golgi network or the late endosome. The pro-protein is the major detectable form on the cell surface, whereas the majority of the protein in the cell is processed. - Information by UniProt
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参照データベース
- Entrez Gene: 8751 Human
- Omim: 605548 Human
- SwissProt: Q13444 Human
- Unigene: 312098 Human
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別名
- A disintegrin and metalloproteinase domain 15 (metargidin) antibody
- A disintegrin and metalloproteinase domain 15 antibody
- ADA15_HUMAN antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling ADAM15 with purified ab124698 at 1:800 dilution (2.34 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.
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Anti-ADAM15 antibody [EPR5619] (ab124698) at 1/1000 dilution (purified) + SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 93 kDa
Observed band size: 110,90 kDa why is the actual band size different from the predicted? -
Anti-ADAM15 antibody [EPR5619] (ab124698) at 1/1000 dilution + SW480 cell lysate at 10 µg
Secondary
Goat anti-Rabbit HRP
Predicted band size: 93 kDaab124698 also appears to recognize the pro-protein (110kDa). This image was produced using unpurified antibody.
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ab124698, at 1/250 dilution, staining ADAM15 in paraffin-embedded Human colon tissue by immunohistochemistry. This image was produced using unpurified antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab124698 は 4 報の論文で使用されています。
- Yang CY et al. Quantitative Proteomics Reveals That ADAM15 Can Have Proteolytic-Independent Functions in the Steady State. Membranes (Basel) 12:N/A (2022). PubMed: 35736286
- Jana S et al. Disparate Remodeling of the Extracellular Matrix and Proteoglycans in Failing Pediatric Versus Adult Hearts. J Am Heart Assoc 7:e010427 (2018). PubMed: 30371322
- Zhang K et al. A novel class of microRNA-recognition elements that function only within open reading frames. Nat Struct Mol Biol 25:1019-1027 (2018). PubMed: 30297778
- Dong DD et al. ADAM15 targets MMP9 activity to promote lung cancer cell invasion. Oncol Rep 34:2451-60 (2015). PubMed: 26323669