Anti-ADAM12 抗体 - Aminoterminal end (ab39155)


  • 製品名Anti-ADAM12 antibody - Aminoterminal end
    ADAM12 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ADAM12 - Aminoterminal end
  • 特異性This antibody recognizes latent unprocessed and processed forms of ADAM12, but does not react with other ADAMs.
  • アプリケーション適用あり: Flow Cyt, ICC/IF, WB, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Pig
  • 免疫原

    Synthetic peptide based on the aminoterminal end of human ADAM12. (Peptide available as ab41204.)

  • ポジティブ・コントロール
    • Cell lysate from human fetal fibroblasts (treated with TNF alpha).



Our Abpromise guarantee covers the use of ab39155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use a concentration of 2 µg/ml. PubMed: 19213876ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.
WB 1/1000 - 1/5000. Predicted molecular weight: 100 kDa. A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.
IHC-Fr Use at an assay dependent concentration. PubMed: 23671573


  • 機能Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.
  • 組織特異性Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas.
  • 配列類似性Contains 1 disintegrin domain.
    Contains 1 EGF-like domain.
    Contains 1 peptidase M12B domain.
  • ドメインThe cysteine-rich domain supports cell adhesion through syndecans and triggers signaling events that lead to beta-1 integrin-dependent cell spreading. In carcinomas cells the binding of this domain to syndecans does not allow the integrin-mediated cell spreading.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻訳後修飾The precursor is cleaved by a furin endopeptidase.
  • 細胞内局在Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A disintegrin and metalloproteinase domain 12 antibody
    • ADA12_HUMAN antibody
    • ADAM 12 antibody
    • ADAM metallopeptidase domain 12 antibody
    • ADAM12 antibody
    • CAR10 antibody
    • Disintegrin and metalloproteinase domain-containing protein 12 antibody
    • MCMP antibody
    • MCMPMltna antibody
    • Meltrin alpha antibody
    • Meltrin-alpha antibody
    • Metalloprotease disintegrin 12 transmembrane antibody
    • MLTN antibody
    • MLTNA antibody
    • OTTHUMP00000046766 antibody
    see all

Anti-ADAM12 antibody - Aminoterminal end 画像

  • Anti-ADAM12 antibody - Aminoterminal end (ab39155) at 1/1000 dilution + Human heart tissue lysate - total protein (ab29431) at 10 µg

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution

    Predicted band size : 100 kDa
    Observed band size : 84 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 117 kDa,42 kDa,50 kDa. We are unsure as to the identity of these extra bands.


Anti-ADAM12 antibody - Aminoterminal end (ab39155) 使用論文

This product has been referenced in:
  • Przewozniak M  et al. Adhesion proteins--an impact on skeletal myoblast differentiation. PLoS One 8:e61760 (2013). WB, IHC-Fr, ICC/IF ; Mouse, Rat . Read more (PubMed: 23671573) »
  • Estrella C  et al. Role of A disintegrin and metalloprotease-12 in neutrophil recruitment induced by airway epithelium. Am J Respir Cell Mol Biol 41:449-58 (2009). Flow Cyt ; Human . Read more (PubMed: 19213876) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (adipose stem cells)
Permeabilization Yes - Triton 0.25%
Specification adipose stem cells
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde

Abcam user community

Verified customer

投稿 Mar 07 2017

On our datasheet for ab39155 we suggest using human fetal fibroblasts as a positive control. Otherwise other positive controls which can be used are: Hela cells, A431 cells or human chondrosarcoma.

Vielen Dank für Ihre Email und für Ihr Interesse an unseren Produkten.
Unserem Wissen nach wurde ab39155 bisher noch nicht in IHC-Fr getestet und auch nicht in dieser Anwendung publiziert.
Falls Sie dies selbst testen möchten, kann ich Ihnen ...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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No problem, I will send the product ab56366.

Could you let me know the purchase order number of ab39155?


Thank you for your email.

Lab has confirmed that the immunogen for ab115203 and ab28747 is indeed corresponds to N-terminal end and is in signal peptide region, so these antibodies might not be suitable.

Regarding ab56366 this antibod...

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I have requested confirmation of immunogen sequence from lab. I will get back to you with the reply soon. Once it gets confirmed I will then be able to suggest the suitable antibody.

Many thanks for having patience.

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital ...

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Thank you for taking the time to provide us with the further details. I apologize for the delay of my reply. I am very sorry, that you are experiencing problems with two of our antibodies. I do actually suspect that you have no signal of Adam12 and the...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (NIH3T3)
Specification NIH3T3
Fixative Paraformaldehyde
Permeabilization Yes - Triton-X-100
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 22°C

Abcam user community

Verified customer

投稿 Dec 17 2008

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