製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab78961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Predicted molecular weight: 100 kDa.

Use purified milk proteins or BSA (2-3%) for blocking, as milk can contain ADAMs and lower sensitivity. Do not dilute the primary antibody in milk; Simple TBS or PBS is recommended. Variable size on gel expected, as there are several processed forms of the protein. Detects band of approximately 116-96 kDa (proforms), with 68 kDa active form predominately.

  • 追加情報Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.
    • 組織特異性Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas.
    • 配列類似性Contains 1 disintegrin domain.
      Contains 1 EGF-like domain.
      Contains 1 peptidase M12B domain.
    • ドメインThe cysteine-rich domain supports cell adhesion through syndecans and triggers signaling events that lead to beta-1 integrin-dependent cell spreading. In carcinomas cells the binding of this domain to syndecans does not allow the integrin-mediated cell spreading.
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • 翻訳後修飾The precursor is cleaved by a furin endopeptidase.
    • 細胞内局在Secreted and Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • A disintegrin and metalloproteinase domain 12 antibody
      • ADA12_HUMAN antibody
      • ADAM 12 antibody
      • ADAM metallopeptidase domain 12 antibody
      • ADAM12 antibody
      • Disintegrin and metalloproteinase domain-containing protein 12 antibody
      • MCMP antibody
      • MCMPMltna antibody
      • Meltrin alpha antibody
      • Meltrin-alpha antibody
      • MLTN antibody
      • MLTNA antibody
      • OTTHUMP00000046766 antibody
      see all

    Anti-ADAM12 antibody (ab78961) 使用論文

    ab78961 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"