製品の概要

  • 製品名Anti-ADAM12 antibody
    ADAM12 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to ADAM12
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: ETLKATKYVE LVIVADNREF QRQGKDLEKV KQRLIEIANH VDKFYRPLNI RIVLVGVEVW NDMDKCSVSQ DPFTSLHEFL DWRKMKLLPR KSHDNAQ, corresponding to amino acids 208-305 of Human ADAM12

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab56366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors.
    • 組織特異性Isoform 1 is expressed in placenta and skeletal, cardiac, and smooth muscle. Isoform 2 seems to be expressed only in placenta or in embryo and fetus. Both forms were expressed in some tumor cells lines. Not detected in brain, lung, liver, kidney or pancreas.
    • 配列類似性Contains 1 disintegrin domain.
      Contains 1 EGF-like domain.
      Contains 1 peptidase M12B domain.
    • ドメインThe cysteine-rich domain supports cell adhesion through syndecans and triggers signaling events that lead to beta-1 integrin-dependent cell spreading. In carcinomas cells the binding of this domain to syndecans does not allow the integrin-mediated cell spreading.
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • 翻訳後修飾The precursor is cleaved by a furin endopeptidase.
    • 細胞内局在Secreted and Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • A disintegrin and metalloproteinase domain 12 antibody
      • ADA12_HUMAN antibody
      • ADAM 12 antibody
      • ADAM metallopeptidase domain 12 antibody
      • ADAM12 antibody
      • Disintegrin and metalloproteinase domain-containing protein 12 antibody
      • MCMP antibody
      • MCMPMltna antibody
      • Meltrin alpha antibody
      • Meltrin-alpha antibody
      • MLTN antibody
      • MLTNA antibody
      • OTTHUMP00000046766 antibody
      see all

    Anti-ADAM12 antibody 画像

    • Western blot against tagged recombinant protein immunogen using ab56366 ADAM12 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

    Anti-ADAM12 antibody (ab56366) 使用論文

    ab56366 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Human Cell lysate - other (Mesenchymal stem cell)
    Gel Running Conditions Reduced Denaturing
    Loading amount 20 µg
    Specification Mesenchymal stem cell
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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    投稿 Aug 19 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"