Anti-ADAM10 抗体 - Cytoplasmic domain (ab39177)

製品の概要

  • 製品名
    Anti-ADAM10 antibody - Cytoplasmic domain
    ADAM10 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ADAM10 - Cytoplasmic domain
  • 特異性
    This antibody is specific for the Cytoplasmic domain of ADAM10 and can detect latent and active forms of the enzyme.It will not recognize the shorter form, lacking the transmembrane and Cytoplasmic domains.
  • アプリケーション
    適用あり: IHC-FoFr, IHC-P, WB, ICC/IF, IPmore details
  • 種交差性
    交差種: Mouse, Cow, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide based on the Cytoplasmic domain of human ADAM10, within the last 30 C-terminus amino acids of the 748 amino acid human sequence.

    (Peptide available as ab41164.)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab39177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr Use at an assay dependent concentration. PubMed: 21325636
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/5000. Detects a band of approximately 98 kDa (predicted molecular weight: 84 kDa).
ICC/IF Use a concentration of 10 µg/ml.
IP Use at an assay dependent concentration. PubMed: 19823572Bovine ADAM10-HA construct

ターゲット情報

  • 機能
    Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-
    -Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis.
  • 組織特異性
    Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver.
  • 配列類似性
    Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
  • ドメイン
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻訳後修飾
    The precursor is cleaved by a furin endopeptidase.
  • 細胞内局在
    Cell membrane. Endomembrane system. Is localized in the plasma membrane but is predominantly expressed in the Golgi apparatus and in released membrane vesicles derived likely from the Golgi.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A disintegrin and metalloprotease domain 10 antibody
    • A disintegrin and metalloproteinase domain 10 antibody
    • AD 10 antibody
    • AD10 antibody
    • AD18 antibody
    • ADA10_HUMAN antibody
    • ADAM 10 antibody
    • ADAM metallopeptidase domain 10 antibody
    • ADAM10 antibody
    • CD 156c antibody
    • CD156c antibody
    • CD156c antigen antibody
    • CDw156 antibody
    • disintegrin and metalloproteinase domain containing protein 10 antibody
    • Disintegrin and metalloproteinase domain-containing protein 10 antibody
    • HsT 18717 antibody
    • HsT18717 antibody
    • Kuz antibody
    • Kuzbanian antibody
    • Kuzbanian protein homolog antibody
    • Kuzbanian, Drosophila, homolog of antibody
    • MADM antibody
    • Mammalian disintegrin metalloprotease antibody
    • Mammalian disintegrin-metalloprotease antibody
    • RAK antibody
    see all

Anti-ADAM10 antibody - Cytoplasmic domain 画像

  • Methanol fixed HeLs cells stained with ab39177 (10 µg/ml) for 1 hour at 22°C. For further details please refer to the Abreview

  • IHC image of ab39177 staining in human normal lymph node formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab39177, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

プロトコール

Anti-ADAM10 antibody - Cytoplasmic domain (ab39177) 使用論文

This product has been referenced in:
  • Atapattu L  et al. An activated form of ADAM10 is tumor selective and regulates cancer stem-like cells and tumor growth. J Exp Med 213:1741-57 (2016). WB, IP . Read more (PubMed: 27503072) »
  • Guo Q  et al. Adam10 Mediates the Choice between Principal Cells and Intercalated Cells in the Kidney. J Am Soc Nephrol N/A:N/A (2014). Read more (PubMed: 24904084) »

See all 6 Publications for this product

Product Wall

Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Denaturing (7.6 %)
Sample
Rat Cell lysate - whole cell (H9c2 embrionic cardiomyocytes)
Specification
H9c2 embrionic cardiomyocytes
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Sep 19 2014

We have issued a free of charge replacement.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (SCC25)
Specification
SCC25
Fixative
Acetone/methanol
Permeabilization
Yes - methanol in fixative
Blocking step
BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Jul 14 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela Cells)
Specification
Hela Cells
Fixative
Methanol
Permeabilization
No
Username

Abcam user community

Verified customer

投稿 Feb 26 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MDA-MB231 human breast cancer cell line)
Loading amount
30000 cells
Specification
MDA-MB231 human breast cancer cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Oct 18 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録