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Synthetic peptide based on the Propeptide domain of human ADAM10. (Peptide available as ab41165.)
Our Abpromise guarantee covers the use of ab39178 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 98 kDa (predicted molecular weight: 84 kDa).
Use dilution 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.
|ICC/IF||Use at an assay dependent concentration.|
Methanol fixed Hela cells stained with ab39178 (10 µg/ml) for 1 hour at 22°C. For further details please refer to the Abreview.