製品の概要

  • 製品名Anti-ADAM10 antibody
    ADAM10 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ADAM10
  • アプリケーション適用あり: IHC-P, WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human ADAM10 aa 732-748.
    Sequence:

    PQRQRPRESYQMGHMRR


    (Peptide available as ab7868)

  • ポジティブ・コントロール
    • Jurkat whole cell lysate
  • 特記事項TNFa converting enzyme.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/500.
WB 1/500 - 1/2000. Predicted molecular weight: 84 kDa.Can be blocked with ADAM10 peptide (ab7868).
ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 2 µg/ml.

ターゲット情報

  • 機能Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-
    -Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis.
  • 組織特異性Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver.
  • 配列類似性Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
  • ドメインThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻訳後修飾The precursor is cleaved by a furin endopeptidase.
  • 細胞内局在Cell membrane. Endomembrane system. Is localized in the plasma membrane but is predominantly expressed in the Golgi apparatus and in released membrane vesicles derived likely from the Golgi.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A disintegrin and metalloprotease domain 10 antibody
    • A disintegrin and metalloproteinase domain 10 antibody
    • AD 10 antibody
    • AD10 antibody
    • ADA10_HUMAN antibody
    • ADAM 10 antibody
    • ADAM metallopeptidase domain 10 antibody
    • ADAM10 antibody
    • CD 156c antibody
    • CD156c antibody
    • CD156c antigen antibody
    • CDw156 antibody
    • disintegrin and metalloproteinase domain containing protein 10 antibody
    • Disintegrin and metalloproteinase domain-containing protein 10 antibody
    • HsT 18717 antibody
    • HsT18717 antibody
    • Kuz antibody
    • Kuzbanian antibody
    • Kuzbanian protein homolog antibody
    • MADM antibody
    • Mammalian disintegrin metalloprotease antibody
    • Mammalian disintegrin-metalloprotease antibody
    see all

Anti-ADAM10 antibody 画像

  • ab1997 at 2µg/ml staining K562 cells by ICC/IF.
  • ICC/IF image of ab1997 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1997, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-ADAM10 antibody (ab1997) at 1/1666 dilution

    Lane 2 : ADAM10 siRNA treated human keratinocyte cells
    Lane 3 : Non-treated human keratinocyte cells.

    Secondary
    HRP conjugated Goat anti-rabbit antibody
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 84 kDa
    Observed band size : 75,100 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Immunofluorescence of ADAM10 in K562 cells using ab1997 at 10 ug/ml.

  • Lane 1 : Anti-ADAM10 antibody (ab1997) at 1 µg/ml
    Lane 2 : Anti-ADAM10 antibody (ab1997) at 2 µg/ml

    Lane 1 : Jurkat whole cell lysate
    Lane 2 : Jurkat whole cell lysate


    Predicted band size : 84 kDa
  • All lanes : Anti-ADAM10 antibody (ab1997) at 1/1000 dilution

    Lane 1 : Human frontal cortex tissue lysate - whole
    Lane 2 : Human frontal cortex tissue lysate - whole

    Lysates/proteins at 20 µg per lane.

    Secondary
    IRDye® 800CW-conjugated donkey anti-rabbit IgG polyclonal at 1/18000 dilution

    Performed under reducing conditions.

    Predicted band size : 84 kDa
    Observed band size : 65,80 kDa (why is the actual band size different from the predicted?)


    Exposure time : 330 seconds

    This image is courtesy of an Abreview submitted by Grant Corbett

    See Abreview

Anti-ADAM10 antibody (ab1997) 使用論文

This product has been referenced in:
  • Cai Z  et al. Activation of cell-surface proteases promotes necroptosis, inflammation and cell migration. Cell Res N/A:N/A (2016). Read more (PubMed: 27444869) »
  • Yan X  et al. Ginsenoside Rd. promotes non-amyloidogenic pathway of amyloid precursor protein processing by regulating phosphorylation of estrogen receptor alpha. Life Sci N/A:N/A (2016). Read more (PubMed: 27825720) »

See all 17 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Monkey Cell (Kidney)
Permeabilization Yes - 0.1% troton x-100
Specification Kidney
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jun 06 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Brain)
Gel Running Conditions Non-reduced Non-Denaturing (Native) (4-12)
Loading amount 30 µg
Specification Brain
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Apr 08 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions Non-reduced Non-Denaturing (Native) (4-12)
Loading amount 40 µg
Specification Kidney
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Apr 08 2016

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Mar 02 2016

Application Immunoprecipitation
Sample Human Cell lysate - whole cell (Melanoma cell line)
Total protein in input 1000 µg
Immuno-precipitation step Protein G
Specification Melanoma cell line
Username

Dr. Praveen Agrawal

Verified customer

投稿 Nov 18 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Spleen and oesophagus)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Spleen and oesophagus
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Sep 23 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Sample Mouse Cell (Neurons)
Specification Neurons
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Nov 04 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (8% in MES)
Sample Human Tissue lysate - whole (Frontal Cortex)
Specification Frontal Cortex
Blocking step Li-Cor Block Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 21°C
Username

Dr. Grant Corbett

Verified customer

投稿 Jul 18 2014

Thank you very much for your call today and for your patience.

I apologize for the delay getting this protocol to you, as it needed to be typed up and re-formatted before sending. Please find the protocol used to test the antibody in ELISA at...

Read More

Thank you and your customerfor taking the time to complete our questionnaire and contact us. I am sorry to hear your customerhas had difficulty obtaining satisfactory results from this antibody.

The details your customerhas kindly provided w...

Read More

1-10 of 16 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"