製品の概要

  • 製品名Anti-AcV5 tag antibody [AcV5]
  • 製品の詳細
    Mouse monoclonal [AcV5] to AcV5 tag
  • 特異性This antibody recognizes a nine amino acid residue tag (SWKDASGWS) of the baculovirus Autographa californica GP64 envelope fusion protein. Proteins tagged with this AcV5 peptide are functional in both dicots and monocots plants and show no cross reactivity across many plant species.
  • アプリケーション適用あり: IP, ICC, Dot blotmore details
  • 種交差性
    Reacts with
  • 免疫原

    AcNPV extracellular nonocculeded virus (NOV)

  • ポジティブ・コントロール
    • Extracts of Sf9 cells infected with baculovirus.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • ポリ/モノモノクローナル
  • クローン名AcV5
  • ミエローマSp2/0-Ag14
  • アイソタイプIgG2b
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab49581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.
Dot blot Use a concentration of 0.5 - 1 µg/ml.

ターゲット情報

  • 関連性Epitope tagged fusion proteins such as AcV5 tag are widely used in protein analysis of biological samples (e.g. plant cell extracts). Antibodies specific for the tag are a valuable means for the detection of the protein. AcV5 recognizes a nine amino acid residue tag of baculovirus Autographa californica GP64 envelope fusion protein (efp). GP64 gene open reading frame encodes a protein of 509 amino acids with a molecular weight of 58 kDa. The protein contains N-linked glycosylation sites and hydrophobic N- and C-termini, characteristic of signal and membrane anchor motifs found in envelope glycoproteins. The GP64 protein is present early (6 hr) post infection and accumulates in the infected cell moving to the periphery later.

Anti-AcV5 tag antibody [AcV5] (ab49581) 使用論文

ab49581 has not yet been referenced specifically in any publications.

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