製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ACTR1A
  • 特異性
    The antibody is specific to a peptide sequence highly conserved (89% identity) in the alpha centractin isoform, and not found in known actin isoforms.
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Dog
  • 免疫原

    Synthetic peptide:

    ACYLSINPQKDETLETEKAQ

    conjugated to KLH, corresponding to amino acids 222-240 of Human ACTR1A.

  • 特記事項
    If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ICC/IF
  • 追加情報
    IF: 1/100.
    For indirect immunofluorescence, a minimum working antibody dilution of 1:100 is recommended using mouse fibroblasts NIH3T cell.
    WB: 1/1000 (recommended using a rat brain cytosol extract and a whole cell extract of the human epidermoid carcinoma A431 cell line). Predicted molecular weight: 45 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Component of a multi-subunit complex involved in microtubule based vesicle motility. It is associated with the centrosome.
    • 配列類似性
      Belongs to the actin family. ARP1 subfamily.
    • 細胞内局在
      Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Actin related protein 1 antibody
      • Actin RPV antibody
      • Actin-RPV antibody
      • ACTR 1A antibody
      • Actr1a antibody
      • ACTZ_HUMAN antibody
      • Alpha centractin antibody
      • Alpha-centractin antibody
      • ARP 1 antibody
      • ARP1 actin related protein 1 homolog A antibody
      • ARP1 actin related protein 1 homolog A centractin alpha antibody
      • ARP1 antibody
      • ARP1 yeast homolog A antibody
      • Centractin alpha antibody
      • Centractin antibody
      • Centrosome associated actin homolog antibody
      • Centrosome-associated actin homolog antibody
      • CTRN 1 antibody
      • CTRN1 antibody
      • FLJ52695 antibody
      • FLJ52800 antibody
      • FLJ55002 antibody
      see all

    Anti-ACTR1A antibody 画像

    • Immunohistochemistry (Formalin fixed, paraffin-embedded sections) analysis of rat duodenum tissue labelling ACTR1A with ab11009.

    • Immunohistochemistry (Formalin fixed, paraffin-embedded sections) analysis of rat kidney tissue labelling ACTR1A with ab11009.

    • All lanes : Anti-ACTR1A antibody (ab11009) at 1/1000 dilution

      Lane 1 : A432 cell lysate
      Lane 2 : A432 cell lysate with ACTR1A immunising peptide at 5 mg/ml


      Predicted band size : 45 kDa
      Observed band size : 45 kDa

    Anti-ACTR1A antibody (ab11009) 使用論文

    ab11009 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Cow Cell lysate - whole cell (Lung)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Lung
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Jun 26 2017

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    African green monkey Cell lysate - whole cell (Kidney)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Kidney
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Jun 26 2017

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Brain)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Jun 26 2017

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Kidney)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Kidney
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Jun 26 2017

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    African green monkey Cell (Kidney)
    Permeabilization
    Yes - 0.2% triton x 100
    Specification
    Kidney
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Jun 15 2017

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - 0.3% triton x 100
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 May 22 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録