Anti-Activin Receptor Type IIB/ACVR2B 抗体 [1C11] (ab76940)
Key features and details
- Mouse monoclonal [1C11] to Activin Receptor Type IIB/ACVR2B
- Suitable for: WB, ELISA, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2a
製品の概要
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製品名
Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11]
Activin Receptor Type IIB/ACVR2B 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [1C11] to Activin Receptor Type IIB/ACVR2B -
由来種
Mouse -
アプリケーション
適用あり: WB, ELISA, Flow Cytmore details -
種交差性
交差種: Human, Recombinant fragment
交差が予測される動物種: Rat, Sheep, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Macaque monkey -
免疫原
Recombinant fragment corresponding to Human Activin Receptor Type IIB/ACVR2B aa 1-150.
Database link: Q13705 -
ポジティブ・コントロール
- Lysate from 293T cells transfected with Activin Receptor Type IIB/ACVR2B
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特記事項
This product was changed from ascites to tissue culture supernatant on 22/03/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
1C11 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76940の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 58 kDa.
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ELISA |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 58 kDa. |
ELISA
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin A, activin B and inhibin A. -
関連疾患
Defects in ACVR2B are the cause of visceral heterotaxy autosomal type 4 (HTX4) [MIM:613751]. A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy type 4 include dextrocardia, right aortic arch and a right-sided spleen, anomalies of the inferior and the superior vena cava, atrial ventricular canal defect with dextro-transposed great arteries, pulmonary stenosis, polysplenia and midline liver. -
配列類似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
Contains 1 protein kinase domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 374213 Chicken
- Entrez Gene: 282131 Cow
- Entrez Gene: 93 Human
- Entrez Gene: 448845 Pig
- Entrez Gene: 25366 Rat
- Omim: 602730 Human
- SwissProt: Q90670 Chicken
- SwissProt: Q95126 Cow
see all -
別名
- Activin A receptor type IIB antibody
- Activin receptor type 2B antibody
- Activin receptor type IIB antibody
see all
画像
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All lanes : Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (ab76940)
Lane 1 : IIB/ACVR2B transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Predicted band size: 58 kDa -
Overlay histogram showing HeLa cells stained with ab76940 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76940, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.This image was generated using the ascites version of the product.
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Anti-Activin Receptor Type IIB/ACVR2B antibody [1C11] (ab76940) at 1/500 dilution + immunogen (GST-tagged 100aa recombinant fragment) at 0.2 µg
Secondary
Goat anti-Mouse IgG (H&L) HRP-conjugated at 1/5000 dilution
Predicted band size: 58 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?MW of GST tag alone: 26 kDa
This image was generated using the ascites version of the product.
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Detection limit for recombinant GST tagged ACVR2B is 0.1 ng/ml as a capture antibody.
データシートおよび資料
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Datasheet download
参考文献 (10)
ab76940 は 10 報の論文で使用されています。
- Toms D et al. Automated Hypothesis Generation to Identify Signals Relevant in the Development of Mammalian Cell and Tissue Bioprocesses, With Validation in a Retinal Culture System. Front Bioeng Biotechnol 8:534 (2020). PubMed: 32582664
- Barrault L et al. Expression of miRNAs from the Imprinted DLK1/DIO3 Locus Signals the Osteogenic Potential of Human Pluripotent Stem Cells. Cells 8:N/A (2019). PubMed: 31779280
- Tinklenberg JA et al. Myostatin Inhibition Using ActRIIB-mFc Does Not Produce Weight Gain or Strength in the Nebulin Conditional KO Mouse. J Neuropathol Exp Neurol 78:130-139 (2019). PubMed: 30597051
- Tinklenberg JA et al. Myostatin inhibition using mRK35 produces skeletal muscle growth and tubular aggregate formation in wild type and TgACTA1D286G nemaline myopathy mice. Hum Mol Genet 27:638-648 (2018). PubMed: 29293963
- Singh R et al. Follistatin Targets Distinct Pathways To Promote Brown Adipocyte Characteristics in Brown and White Adipose Tissues. Endocrinology 158:1217-1230 (2017). PubMed: 28324027
- Tinklenberg J et al. Treatment with ActRIIB-mFc Produces Myofiber Growth and Improves Lifespan in the Acta1 H40Y Murine Model of Nemaline Myopathy. Am J Pathol 186:1568-81 (2016). PubMed: 27102768
- Kornegay JN et al. Dystrophin-deficient dogs with reduced myostatin have unequal muscle growth and greater joint contractures. Skelet Muscle 6:14 (2016). PubMed: 27047655
- Choi YJ et al. Identifying an ovarian cancer cell hierarchy regulated by bone morphogenetic protein 2. Proc Natl Acad Sci U S A 112:E6882-8 (2015). PubMed: 26621735
- Lawlor MW et al. Differential muscle hypertrophy is associated with satellite cell numbers and Akt pathway activation following activin type IIB receptor inhibition in Mtm1 p.R69C mice. Am J Pathol 184:1831-42 (2014). PubMed: 24726641
- Perron JC & Dodd J Inductive specification and axonal orientation of spinal neurons mediated by divergent bone morphogenetic protein signaling pathways. Neural Dev 6:36 (2011). PubMed: 22085733