Anti-Activin Receptor Type IIA 抗体 (ab10595)

製品の概要

  • 製品名Anti-Activin Receptor Type IIA antibody
    Activin Receptor Type IIA 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Activin Receptor Type IIA
  • 特異性The antibody exhibits less than 2 % cross-reactivity with recombinant human activin receptor I and recombinant human activin receptor IIB.
  • アプリケーション適用あり: IHC-P, Indirect ELISA, IHC-Fr, WB, Neutralisingmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant fragment (Human)from the Activin RIIA extracellular domain.

  • 特記事項


    Activin, a disulfide-linked homodimeric protein is secreted by Sertoli cells in the testis and granulosa cells in the ovary. In early studies, this peptide was thought to be an inhibin and not recognized as a unique compound. Activins and inhibins are members of the TGF-beta superfamily due to amino acid homology with respect to the conservation of 7 of the 9 cysteine residues common to all TGF-beta forms. Activins are homodimers or heterodimers of the various beta subunit isoforms, while inhibins are heterodimers of a unique alpha subunit and one of the various beta subunits. Five beta subunits have been cloned (mammalian betaA, betaB, betaC, betaE, and Xenopus betaD). The activin/inhibin nomenclature reflects the subunit composition of the proteins: activin A (betaA-betaA), activin B (betaB-betaB), activin AB (betaB-betaA), inhibin A (alpha-betaA), and inhibin B (alpha- betaB). Activins have a wide range of biological activities including mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis, and reproductive physiology. Activins are also involved in growth and differentiation of several tissues from different species. This protein also plays a key role in the production and regulation of hormones such as FSH, LH, GnRH, and ACTH. Activin influences erythropoiesis and the potentiation of erythroid colony formation, oxytocin secretion, paracrine, and autocrine regulation. Similar to other TGF-beta family members, activins exert their biological activities through the effects ot the heterodimeric complex composed of two membrane spanning serine-threonine kinases designated type I and type receptors. Activin type I and type II receptors are distinguished by the level of sequence homology of their kinase domains and other structural and functional features. To date, seven type I and five type II activin receptors have been cloned from mammals, including activin receptor IA, activin receptor IIA, activin receptor IB, and activin receptor IIB. In addition, two splice variants of activin receptor IIA and five splice variants of activin receptor IIB have been reported. Type I activin receptors are highly conserved and do not bind directly to activin but will associate with the type II receptor-activin complex and initiate signal transduction. Type I activin receptors will also bind with the BMP-2/7-bound BMPR-II and form signaling complexes. Human, mouse, and bovine type IB activin receptors share greater than 98 % homology.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 5% Trehalose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 一次抗体 備考Activin, a disulfide-linked homodimeric protein is secreted by Sertoli cells in the testis and granulosa cells in the ovary. In early studies, this peptide was thought to be an inhibin and not recognized as a unique compound. Activins and inhibins are members of the TGF-beta superfamily due to amino acid homology with respect to the conservation of 7 of the 9 cysteine residues common to all TGF-beta forms. Activins are homodimers or heterodimers of the various beta subunit isoforms, while inhibins are heterodimers of a unique alpha subunit and one of the various beta subunits. Five beta subunits have been cloned (mammalian betaA, betaB, betaC, betaE, and Xenopus betaD). The activin/inhibin nomenclature reflects the subunit composition of the proteins: activin A (betaA-betaA), activin B (betaB-betaB), activin AB (betaB-betaA), inhibin A (alpha-betaA), and inhibin B (alpha- betaB). Activins have a wide range of biological activities including mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis, and reproductive physiology. Activins are also involved in growth and differentiation of several tissues from different species. This protein also plays a key role in the production and regulation of hormones such as FSH, LH, GnRH, and ACTH. Activin influences erythropoiesis and the potentiation of erythroid colony formation, oxytocin secretion, paracrine, and autocrine regulation. Similar to other TGF-beta family members, activins exert their biological activities through the effects ot the heterodimeric complex composed of two membrane spanning serine-threonine kinases designated type I and type receptors. Activin type I and type II receptors are distinguished by the level of sequence homology of their kinase domains and other structural and functional features. To date, seven type I and five type II activin receptors have been cloned from mammals, including activin receptor IA, activin receptor IIA, activin receptor IB, and activin receptor IIB. In addition, two splice variants of activin receptor IIA and five splice variants of activin receptor IIB have been reported. Type I activin receptors are highly conserved and do not bind directly to activin but will associate with the type II receptor-activin complex and initiate signal transduction. Type I activin receptors will also bind with the BMP-2/7-bound BMPR-II and form signaling complexes. Human, mouse, and bovine type IB activin receptors share greater than 98 % homology.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab10595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 15 µg/ml.
Indirect ELISA Use a concentration of 0.5 - 1 µg/ml.
IHC-Fr Use at an assay dependent dilution. PubMed: 16835628
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 58 kDa. ~5 ng/lane under reducing and non-reducing conditions.
Neutralising Use a concentration of 1 - 3 µg/ml. The antibody will block 50% of the binding of 30 ng/ml recombinant human activin A to immobilized recombinant human activin receptor IIA/Fc chimera (100 µl of a 0.5 µg/ml solution coating each well) in a functional ELISA assay. ab10595 has not been optimized for cell/tissue culture conditions.

ターゲット情報

  • 機能On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin A, activin B and inhibin A.
  • 配列類似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
    Contains 1 protein kinase domain.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Activin A receptor type IIA antibody
    • Activin receptor type 2A antibody
    • Activin receptor type IIA antibody
    • Activin receptor type-2A antibody
    • ACTR 2 antibody
    • ACTR IIA antibody
    • ACTR-IIA antibody
    • ACTR2 antibody
    • ACTRII antibody
    • ACTRIIA antibody
    • Acvr 2 antibody
    • Acvr 2A antibody
    • Acvr2 antibody
    • ACVR2A antibody
    • AVR2A_HUMAN antibody
    • OTTHUMP00000197918 antibody
    see all

Anti-Activin Receptor Type IIA antibody (ab10595) 使用論文

This product has been referenced in:
  • Bauer J  et al. Activin and TGFß use diverging mitogenic signaling in advanced colon cancer. Mol Cancer 14:182 (2015). IHC-P ; Human . Read more (PubMed: 26497569) »
  • Liu XS  et al. Stroke induces gene profile changes associated with neurogenesis and angiogenesis in adult subventricular zone progenitor cells. J Cereb Blood Flow Metab 27:564-74 (2007). IHC-Fr ; Mouse . Read more (PubMed: 16835628) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (12% Gel)
Sample Mouse Tissue lysate - whole (Mouse Testis and Ovary. COV434 human cell line)
Specification Mouse Testis and Ovary. COV434 human cell line
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
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Dr. FRANCESCO ELIA MARINO

Verified customer

投稿 Dec 23 2014

Thank you for your call and for your patience.

Finally, I have some more information regarding the immunogen/epitopes of the the two antibodies you are interested in:


The immunogen used to raise ab10595 Anti-Activin Receptor Type...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. The following publication makes reference to the use of rat ovary as a positive control for IHC and RT-PCR analysis of Activin Receptor Type I...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"