リコンビナント
RabMAb

Anti-Activin A Receptor Type IB 抗体 [EPR4815] (ab109300)

製品の概要

  • 製品名
    Anti-Activin A Receptor Type IB antibody [EPR4815]
    Activin A Receptor Type IB 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4815] to Activin A Receptor Type IB
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    corresponding to Human Activin A Receptor Type IB.

  • ポジティブ・コントロール
    • Human, mouse and rat brain tissue lysate; Human fetal kidney and liver tissue lysate; Human cervical carcinoma; Human kidney tissue; SH-SY5Y and U87-MG cell lysate.
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 57 kDa).
IHC-P 1/1600. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

(Heat to 98°C, allow to cool for 10-20 minutes)

For Unpurified use at 1/500 - 1/1000.

ICC/IF 1/100 - 1/250.
  • 追加情報
    Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能
      On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Phosphorylates TDP2.
    • 組織特異性
      Expressed in many tissues, most strongly in kidney, pancreas, brain, lung, and liver.
    • 配列類似性
      Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
      Contains 1 GS domain.
      Contains 1 protein kinase domain.
    • 翻訳後修飾
      Autophosphorylated.
    • 細胞内局在
      Membrane.
    • Information by UniProt
    • 参照データベース
    • 製品の状態
      There are 3 isoforms produced by alternative splicing. Isoform 1 also known as SKR2-1; isoform 2 as SKR2-2; isoform 3 as SKR2-3.
    • 別名
      • Activin A receptor type 1B antibody
      • Activin A receptor type II like kinase 4 antibody
      • Activin A type 1B receptor antibody
      • Activin A type IB receptor antibody
      • Activin receptor like kinase 4 antibody
      • Activin receptor type 1B antibody
      • Activin receptor type IB antibody
      • Activin receptor type-1B antibody
      • Activin receptor-like kinase 4 antibody
      • ACTR IB antibody
      • ACTR-IB antibody
      • ACTRIB antibody
      • ACV1B_HUMAN antibody
      • ACVR 1B antibody
      • Acvr1b antibody
      • ACVRLK 4 antibody
      • ACVRLK4 antibody
      • ALK 4 antibody
      • ALK-4 antibody
      • ALK4 antibody
      • Serine(threonine) protein kinase receptor R2 antibody
      • Serine/threonine-protein kinase receptor R2 antibody
      • SKR 2 antibody
      • SKR2 antibody
      see all

    画像

    • Anti-Activin A Receptor Type IB antibody [EPR4815] (ab109300) at 1/1000 dilution (purified) + Human fetal kidney lysate at 15 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size : 57 kDa
      Observed band size : 52 kDa (why is the actual band size different from the predicted?)
      Blocking/Diluting buffer 5% NFDM/TBST
    • Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma sections labelling Activin A Receptor Type IB with purified ab109300 at dilution of 1/600. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was heat mediated using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

    • Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labelling Activin A Receptor Type IB with purified ab109300 at 1/250 (5.0 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000, 2 μg/mL) was used as the secondary antibody. Cells were counter-stained with ab195889 Anti-Alpha Tubulin antibody [DM1A] (1/200, 2.5 μg/mL) - Microtubule Marker (Alexa Fluor® 594). DAPI (blue) was used as a nuclear counterstain.

      Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    • All lanes : Anti-Activin A Receptor Type IB antibody [EPR4815] (ab109300) at 1/1000 dilution (purified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 57 kDa
      Observed band size : 52 kDa (why is the actual band size different from the predicted?)
      Blocking/Diluting buffer 5% NFDM/TBST
    • All lanes : Anti-Activin A Receptor Type IB antibody [EPR4815] (ab109300) at 1/1000 dilution (unpurified)

      Lane 1 : Human brain tissue lysate
      Lane 2 : Human fetal kidney
      Lane 3 : SH-SY5Y cell lysate
      Lane 4 : U87-MG cell lysate
      Lane 5 : Human fetal liver

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 57 kDa
      Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of human kidney tissue sections labelling Activin A receptor type IB with unpurified ab109300 at 1/500 dilution. 

    • Immunocytochemistry/ Immunofluorescence analysis of 2% paraformaldehyde fixed (20 mins) SH-SY5Y cells labelling Activin A Receptor Type IB with unpurified ab109300 at a dilution of 1/100. Alexa Fluor488 goat anti-rabbit IgG was used as the secondary antibody. 

    参考文献

    This product has been referenced in:
    • Zhang L  et al. Neuron-derived CCL2 contributes to microglia activation and neurological decline in hepatic encephalopathy. Biol Res 50:26 (2017). WB ; Rat . Read more (PubMed: 28870240) »
    • Lau B  et al. The Expression of Human Cytomegalovirus MicroRNA MiR-UL148D during Latent Infection in Primary Myeloid Cells Inhibits Activin A-triggered Secretion of IL-6. Sci Rep 6:31205 (2016). SDS-PAGE . Read more (PubMed: 27491954) »

    See all 4 Publications for this product

    レビューと Q&A

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    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (eye)
    Specification
    eye
    Fixative
    Formaldehyde
    Permeabilization
    No
    Blocking step
    0.5% BSA + 5% Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    Verified customer

    投稿 Mar 12 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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