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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Overview
Product name
active Caspase 3 peptide
active Caspase 3 抗体 (3件) 一覧
Protein description
Synthetic peptide derived from within residues 150 - 250 of Human active Caspase 3.(Note: the amino acid sequence is proprietary)This peptide was used as an immunogen for ab13847 - active Caspase 3 antibody and ab77973 - active Caspase 3 antibody.
Protein length
277 amino acids
Expression host
CHO
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Information available upon request.
Concentration
Concentration information loading...
Research areas
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
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Western blot
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab13848 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking
Application notes
BL: Use at a concentration of 1 µg/ml.
This peptide can be used with studies using ab13847.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Relevance
The caspase family of cysteine proteases play a key role in apoptosis. Caspase 3 is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as inactive pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). The processed form of Caspase 3 consists of large (17kDa) and small (12kDa) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 Ser29 and Asp175 Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Alternative splicing of this gene results in two transcript variants which encode the same protein. In immunohistochemical studies Caspase 3 expression has been shown to be widespread but not present in all cell types (e.g. commonly reported in epithelial cells of skin, renal proximal tubules and collecting ducts). Differences in the level of Caspase 3 have been reported in cells of short lived nature (eg germinal centre B cells) and those that are long lived (eg mantle zone B cells). Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
Cellular localization
Cytoplasmic
active Caspase 3 peptide images:
Western blot
All lanes : Anti-active Caspase 3 antibody (ab13847) at 1/500 dilution
Lane 1 : Hela whole cell lysate at 20 µg
Lane 2 : A431 whole cell lysate at 20 µg
Lane 3 : Jurkat whole cell lysate at 20 µg
Lane 4 : Hela whole cell lysate at 20 µg with active Caspase 3 peptide (ab13848) at 1 µg/ml
Lane 5 : A431 whole cell lysate at 20 µg with active Caspase 3 peptide (ab13848) at 1 µg/ml
Lane 6 : Jurkat whole cell lysate at 20 µg with active Caspase 3 peptide (ab13848) at 1 µg/ml
Lane 7 : Recombinant Human caspase 3 (active) recombinant protein at 0.1 µg
Lane 8 : Recombinant Human caspase 3 (active) recombinant protein at 0.1 µg with active Caspase 3 peptide (ab13848) at 1 µg/ml
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/5000 dilution
developed using the ECL technique
Predicted band size : 17 kDa
Lanes 1-6: Exposure time 5 min.
Lanes 7-8: Exposure time 30 sec.
ab13847 detects a 17kDa band when tested on recombinant active Caspase 3. This was produced by combining the 17kDa and 12kDa subunits produced separately as recombinant proteins. ab13847 also detects a 17kDa band on lysates from HeLa, A431 and Jurkat cells. These bands can be blocked by the immunising peptide. This strongly suggests that ab13847 is specific for active Caspase 3.
References for active Caspase 3 peptide (ab13848)
ab13848 has not yet been referenced specifically in any publications.
Publishing research using ab13848? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"