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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Overview
Product name
Anti-active Caspase 3 antibody
active Caspase 3 抗体 (4件) 一覧
Description
Rabbit polyclonal to active Caspase 3
Specificity
The antibody preferentially recognizes the p17 fragment of the active Caspase 3. Under the conditions we tested, the antibody did not detect the precursor form. Under other conditions (such as those used in PMC2206181), this antibody has been found to detect both the pro and active forms of Caspase 3.
Tested applications
IHC-FoFr, IHC-P, IHC-Fr, WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Quail
Immunogen
Synthetic peptide corresponding the cleavage site of human caspase-3.(Peptide available as ab38283.)
Positive control
Rat brain,rat foetus, mouse foetus (day 9)(head, heart) (see reference Mirkes et al). Camptothecin (2 µM) treated Jurkat cells. If no signal is observed a time course may be required to identify maximal caspase activity.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 30% Glycerol, 0.5% BSA, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 3 has been extensively studied and implicated to play an important role in apoptosis. Active caspase 3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP). This affinity purified antibody recognizing the active forms of caspase-3 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2302 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FoFr: Use at an assay dependent dilution. (PubMed: 18797916)
IHC-P: Use a concentration of 10 - 20 µg/ml.Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
IHC-Fr: Use a concentration of 10 - 20 µg/ml.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 17 kDa.
ICC/IF: 1/200(PubMed: 17003475 For a positive signal the cells or tissues need to contain apoptotic cells and caspase-3 has to be activated. )
Target
Relevance
The caspase family of cysteine proteases play a key role in apoptosis. Caspase 3 is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as inactive pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). The processed form of Caspase 3 consists of large (17kDa) and small (12kDa) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 Ser29 and Asp175 Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Alternative splicing of this gene results in two transcript variants which encode the same protein. In immunohistochemical studies Caspase 3 expression has been shown to be widespread but not present in all cell types (e.g. commonly reported in epithelial cells of skin, renal proximal tubules and collecting ducts). Differences in the level of Caspase 3 have been reported in cells of short lived nature (eg germinal centre B cells) and those that are long lived (eg mantle zone B cells). Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
Cellular localization
Cytoplasmic
Alternative names
- APOPAIN antibody
- CASP3 antibody
- Caspase 3 apoptosis related cysteine protease antibody
see all
Database links
- Entrez Gene: 836 Human
- Entrez Gene: 12367 Mouse
- Entrez Gene: 25402 Rat
- Omim: 600636 Human
- SwissProt: P42574 Human
- SwissProt: P70677 Mouse
- SwissProt: P55213 Rat
- Unigene: 141125 Human
see all
Anti-active Caspase 3 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - active Caspase 3 antibody (ab2302)
Lung sections were stained with caspase antibody ab2302. Caspase 3 positive cells are shown in brown (DAB). This picture was kindly supplied as part of a customer review.
Western blot - active Caspase 3 antibody (ab2302)
Anti-active Caspase 3 antibody (ab2302) at 1 µg/ml + Camptothecin (2 µM) treated Jurkat cells.
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - active Caspase 3 antibody (ab2302)
ab2302 at a 1/20 dilution staining active caspase 3 from rat pancreas tissue sections by IHC-P. Heat mediated antigen retrieval was used and the tissue was formaldehyde fixed and blocked with 20% serum. The primary antibody was then incubated with the tissue for 30 minutes. A HRP conjugated goat polyclonal was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - active Caspase 3 antibody (ab2302)
ab2302 at 1/50 staining mouse embryo (day 16) tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed in citric acid. The tissue was then blocked and incubated with the antibody for 1 hour. A biotinylated goat anti-rabbit antibody was used as the secondary.
The image shows an E16 mouse body parasaggital sections showinf DRGs in the lumbar/sacral region of the spinal column (there is highest apoptotic index in the organ at this age).
This image is courtesy of an Abreview submitted by Mr Carl hobbs
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - active Caspase 3 antibody (ab2302)
Immunohistochemistical detection (on formaldehyde/PFA-fixed paraffin-embedded sections) of active Caspase 3 antibody (ab2302) on Quail Tissue sections (Quail E6/7 developing DRGs Saggital section). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins RT. Primary Antibody ab2302 incubated at 1/50 for at RT. Secondary Antibody: Biotin conjugated goat anti rabbit IgG (1/300).
Carl Hobbs, CARD, KCL, London, UK
References for Anti-active Caspase 3 antibody (ab2302)
This product has been referenced in:
- Melo FRet al. A Role for Serglycin Proteoglycan in Mast Cell Apoptosis Induced by a Secretory Granule-mediated Pathway. J Biol Chem 286:5423-33 (2011).Read more (PubMed: 21123167) »
- Ho TCet al. Pigment Epithelium-derived Factor (PEDF) Promotes Tumor Cell Death by Inducing Macrophage Membrane Tumor Necrosis Factor-related Apoptosis-inducing Ligand (TRAIL). J Biol Chem 286:35943-54 (2011). WB.Read more (PubMed: 21846721) »
See all 45 publications for this product
Publishing research using ab2302? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"