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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - active Caspase 3 antibody (ab2302)

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Western blot - active Caspase 3 antibody (ab2302)

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Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - active Caspase 3 antibody (ab2302)

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Product Name
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active Caspase 3 antibody
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active Caspase 3 抗体 (4件) 一覧
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to active Caspase 3
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Immunogen
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Synthetic peptide corresponding the cleavage site of human caspase-3.(Peptide available as ab38283.)
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Reacts with
(species key)
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Hu, Ms, Rat, Ql
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Specificity
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The antibody preferentially recognizes the p17 fragment of the active Caspase 3 (under the condition we tested, the antibody did not detect the precursor form and based on the purification method we used the antibody should not detect any precursor form).
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Tested applications
(see key)
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ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions IHC-FoFr: Use at an assay dependent dilution (PMID 18797916). IHC-P: Suggested working dilution of 1/50 (see PMID: 16202037). In addition, found to work at 1/100 (see PMID: 15885730). For antigen unmasking, heat sections in 10 mM sodium citrate buffer (pH 6.0) for 1 minute at full power followed by 9 minutes at medium power. (Keep slides fully immersed in buffer and maintain temperature at or just below boiling.) Cool slides for 20 minutes after antigen unmasking. IHC-Fr: Use at a concentration of 10 - 20 µg/ml. WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 17-20 kDa. ICC/IF: 1/200 (From PubMed:17003475).
For a positive signal the cells or tissues need to contain apoptotic cells and caspase-3 has to be activated. The recommended dilutions provide only guidelines. More antibody should be applied if very little caspase-3 has been expressed.
Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
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Rat brain,rat foetus, mouse foetus (day 9)(head, heart) (see reference Mirkes et al). Camptothecin (2 µM) treated Jurkat cells. If no signal is observed a time course may be required to identify maximal caspase activity.
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Cellular localization
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Cytoplasmic
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Research areas
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Neuroscience >> Neurology process >> Neurodegenerative disease >> Other Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
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Relevance
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The caspase family of cysteine proteases play a key role in apoptosis. Caspase 3 is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as inactive pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). The processed form of Caspase 3 consists of large (17kDa) and small (12kDa) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 Ser29 and Asp175 Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Alternative splicing of this gene results in two transcript variants which encode the same protein. In immunohistochemical studies Caspase 3 expression has been shown to be widespread but not present in all cell types (e.g. commonly reported in epithelial cells of skin, renal proximal tubules and collecting ducts). Differences in the level of Caspase 3 have been reported in cells of short lived nature (eg germinal centre B cells) and those that are long lived (eg mantle zone B cells). Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 3 has been extensively studied and implicated to play an important role in apoptosis. Active caspase 3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP). This affinity purified antibody recognizing the active forms of caspase-3 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
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active Caspase 3 antibody (ab2302)の別名
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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PBS with 1 mg/ml BSA, 1.5 mM sodium azide and 50% glycerol Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Abcam では全ての製品情報を一元管理しており、 active Caspase 3 antibody について私たちが知りうる全ての情報がこのデータシートでご覧いただけます。ご不明な点がありましたら、 お問い合わせください。 |
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See below for active Caspase 3 antibody images, references, products related to ab2302 and other tools.
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active Caspase 3 antibody images:
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - active Caspase 3 antibody (ab2302)
Lung sections were stained with caspase antibody ab2302. Caspase 3 positive cells are shown in brown (DAB). This picture was kindly supplied as part of a customer review.
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Western blot - active Caspase 3 antibody (ab2302)
active Caspase 3 antibody (ab2302) at 1 µg/ml + Camptothecin
(2 µM) treated Jurkat cells.
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Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - active Caspase 3 antibody (ab2302)
ab2302 at a 1/20 dilution staining active caspase 3 from rat pancreas tissue sections by IHC-P. Heat mediated antigen retrieval was used and the tissue was formaldehyde fixed and blocked with 20% serum. The primary antibody was then incubated with the tissue for 30 minutes. A HRP conjugated goat polyclonal was used as the secondary antibody. This image is courtesy of an anonymous Abreview
See Abreview
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - active Caspase 3 antibody (ab2302)
ab2302 at 1/50 staining mouse embryo (day 16) tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed in citric acid. The tissue was then blocked and incubated with the antibody for 1 hour. A biotinylated goat anti-rabbit antibody was used as the secondary. The image shows an E16 mouse body parasaggital sections showinf DRGs in the lumbar/sacral region of the spinal column (there is highest apoptotic index in the organ at this age). This image is courtesy of an Abreview submitted by Mr Carl hobbs
See Abreview
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - active Caspase 3 antibody (ab2302)
Immunohistochemistical detection (on formaldehyde/PFA-fixed paraffin-embedded sections) of active Caspase 3 antibody (ab2302) on Quail Tissue sections (Quail E6/7 developing DRGs Saggital section). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins RT. Primary Antibody ab2302 incubated at 1/50 for at RT. Secondary Antibody: Biotin conjugated goat anti rabbit IgG
(1/300). Carl Hobbs, CARD, KCL, London, UK
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References for active Caspase 3 antibody (ab2302)
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This product has been used in: (two most recent references)
Martinez EC et al. Ascorbic acid improves embryonic cardiomyoblast cell survival and promotes vascularization in potential myocardial grafts in vivo. Tissue Eng Part A 16:1349-61 (2010). ICC/IF; Rat. PubMed: 19908964
Zehir A et al. Dicer is required for survival of differentiating neural crest cells. Dev Biol 340:459-67 (2010). PubMed: 20144605
See all 42 publication references for this product.
If you publish research using ab2302 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to active Caspase 3
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