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Synthetic peptide corresponding to Human activated Notch1 aa 1720-1770.
A VQSETVEPPP PAQLHFMYVA AAAFVLLFFV GCGVLLSRKR RRQHGQLWFP
ab52301 is cleaved at Val 1744
Our Abpromise guarantee covers the use of ab52301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 20680961|
|IHC-P||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 272 kDa).|
Immunofluorescence analysis of frozen tissue sections (10µm) of intestine from sham operated rats (control group) or rats after Ischemic-Reperfusion (I/R) injury labeling Notch1 with ab52301. Tissue sections were frozen after being fixed with 4% formaldehyde, then incubated in 3% H2O2 for 30 minutes and then blocked with 5% BSA for 30 mins at room temperature. The sections were incubated overnight with ab52301 (PBS + 3% BSA). An appropriate secondary antibody was then added. DAPI (in blue) was used to stain the nucleus.
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