製品の概要

  • 製品名Anti-Actin antibody [MAC 237]
    Actin 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [MAC 237] to Actin
  • 特異性Antibody reacts with actin in Lethocerus and Drosophila flight and non-flight muscle and with actin in flight muscles of all other insect species tested. Also reacts with mammalian actin (tested vs. rat). Antibody reacts with arthrin in flight muscles of Hemiptera and Diptera.
  • アプリケーション適用あり: IHC-P, ICC/IF, Electron Microscopymore details
  • 種交差性
    交差種: Mouse, Rat, Human, Drosophila melanogaster, Lethocerus indicus (Waterbug)
  • 免疫原

    Flight muscle extract from Lethocerus indicus (Waterbug)

  • ポジティブ・コントロール
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal skin.

アプリケーション

Our Abpromise guarantee covers the use of ab50591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
Electron Microscopy Use at an assay dependent concentration.

ターゲット情報

  • 機能Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • 関連疾患Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • 配列類似性Belongs to the actin family.
  • 細胞内局在Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 参照データベース
  • 別名
    • a actin antibody
    • ACTA antibody
    • ACTA1 antibody
    • Actin alpha skeletal muscle antibody
    • Actin antibody
    • actin, alpha 1, skeletal muscle 1 antibody
    • actin, alpha 1, skeletal muscle antibody
    • Actin, alpha skeletal muscle antibody
    • actina antibody
    • actine antibody
    • ACTS_HUMAN antibody
    • aktin antibody
    • Alpha Actin 1 antibody
    • Alpha skeletal muscle Actin antibody
    • alpha skeletal muscle antibody
    • alpha-actin antibody
    • Alpha-actin-1 antibody
    • ASMA antibody
    • CFTD antibody
    • CFTD1 antibody
    • CFTDM antibody
    • MPFD antibody
    • NEM1 antibody
    • NEM2 antibody
    • NEM3 antibody
    • nemaline myopathy type 3 antibody
    see all

Anti-Actin antibody [MAC 237] 画像

  • ab50591 staining Actin in mouse proximal colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% NDS for 24 hours at 4°C; antigen retrieval was by heat mediation in buffer, pH9. Samples were incubated with primary antibody (1/100 in 5% BSA/NDS) for 24 hours at 4°C. An undiluted Alexa Fluor® 647-conjugated donkey anti-rat IgG polyclonal was used as the secondary antibody.

    Also stained with ab76055 (green) at 1/100.

    See Abreview

  • IHC image of Actin staining in Human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab50591, 5µg/ml, for 15 mins at room temperature. A Goat anti-Rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Actin antibody [MAC 237] (ab50591) 使用論文

This product has been referenced in:
  • Shaw WR  et al. Mating activates the heme peroxidase HPX15 in the sperm storage organ to ensure fertility in Anopheles gambiae. Proc Natl Acad Sci U S A 111:5854-9 (2014). WB . Read more (PubMed: 24711401) »
  • Idrissi FZ  et al. Ultrastructural dynamics of proteins involved in endocytic budding. Proc Natl Acad Sci U S A 109:E2587-94 (2012). Electron Microscopy ; Saccharomyces cerevisiae . Read more (PubMed: 22949647) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Proximal Colon)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: steamer Dako retrivel buffer pH 9
Permeabilization No
Specification Proximal Colon
Blocking step Serum as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative Formaldehyde
Username

Miss. Anne Sailer

Verified customer

投稿 Jul 27 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"