製品の概要

  • 製品名Anti-ACSL3 antibody
    ACSL3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ACSL3
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Orangutan
  • 免疫原

    Synthetic peptide: NSLDGLASVL YPGCDTLDKV FTYAKNKFKN KRLLGTREVL NEEDEVQPNG , corresponding to N terminal amino acids 72-121 of Human ACSL3

  • ポジティブ・コントロール
    • 293T cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab80675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/200.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Acyl-CoA synthetases (ACSL) activates long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis (By similarity). Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates (By similarity). Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins).
  • 配列類似性Belongs to the ATP-dependent AMP-binding enzyme family.
  • 細胞内局在Mitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ACS3 antibody
    • Acsl3 antibody
    • ACSL3_HUMAN antibody
    • Acyl CoA synthetase long chain family member 3 antibody
    • FACL3 antibody
    • Fatty acid Coenzyme A ligase long chain 3 antibody
    • LACS 3 antibody
    • LACS3 antibody
    • Lignoceroyl CoA synthase antibody
    • Long chain acyl CoA synthetase 3 antibody
    • Long chain fatty acid CoA ligase 3 antibody
    • Long-chain acyl-CoA synthetase 3 antibody
    • Long-chain-fatty-acid--CoA ligase 3 antibody
    • PRO2194 antibody
    see all

Anti-ACSL3 antibody 画像

  • Anti-ACSL3 antibody (ab80675) at 1 µg/ml + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 80 kDa
    Observed band size : 80 kDa
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration: 12%
  • Application: IHC. Species+tissue/cell type:THP-1 derived macrophage activated with iMtb. Primary antibody dilution: 1:200. Secondary antibody: Goat anti-rabbit Alexa Fluor 647. Secondary antibody dilution:1:333
  • Application: IHC. Species+tissue/cell type:THP-1 derived macrophage. Primary antibody dilution: 1:200. Secondary antibody: Goat anti-rabbit Alexa Fluor 647. Secondary antibody dilution:1:333

Anti-ACSL3 antibody (ab80675) 使用論文

ab80675 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"