Acyl CoA synthetase long chain family member 1 antibody
Acyl-CoA synthetase 1 antibody
FACL 1 antibody
FACL 2 antibody
Fatty acid Coenzyme A ligase long chain 1 antibody
Fatty acid Coenzyme A ligase long chain 2 antibody
LACS 1 antibody
LACS 2 antibody
Lignoceroyl CoA synthase antibody
Long chain acyl CoA synthetase 1 antibody
Long chain acyl CoA synthetase 2 antibody
Long chain fatty acid CoA ligase 1 antibody
Long chain fatty acid CoA ligase 2 antibody
Long chain fatty acid coenzyme A ligase 1 antibody
Long-chain acyl-CoA synthetase 1 antibody
Long-chain acyl-CoA synthetase 2 antibody
Long-chain fatty acid-CoA ligase 2 antibody
Long-chain-fatty-acid--CoA ligase 1 antibody
Palmitoyl CoA ligase 1 antibody
Palmitoyl CoA ligase 2 antibody
Palmitoyl-CoA ligase 1 antibody
Palmitoyl-CoA ligase 2 antibody
Paltimoyl CoA ligase 1 antibody
Western blot - ACSL1 antibody (ab76702)
All lanes : Anti-ACSL1 antibody (ab76702) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889) Lane 2 : Human kidney tissue lysate - total protein (ab30203) Lane 3 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 78 kDa Observed band size: 78 kDa Additional bands at: 75 kDa (possible isoform)
Exposure time: 3 minutes
This antibody is predicted to recognize isoforms 1 and 2 of ACSL1. ab76702 detects a strong band at approximately 75-kDa in human liver tissue lysate. It is thought that this band corresponds to isoform 2 of the protein (SwissProt data).
ICC/IF image of ab76702 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76702, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Singh AB et al. SREBP2 Activation Induces Hepatic Long-chain Acyl-CoA Synthetase 1 (ACSL1) Expression in Vivo and in Vitro through a Sterol Regulatory Element (SRE) Motif of the ACSL1 C-promoter. J Biol Chem291:5373-84 (2016).
Read more (PubMed: 26728456) »
Kan CF et al. PPARd activation induces hepatic long-chain acyl-CoA synthetase 4 expression in vivo and in vitro. Biochim Biophys Acta1851:577-87 (2015).
Read more (PubMed: 25645621) »