製品の概要

  • 製品名Anti-Acrosin antibody [ACR-2]
    Acrosin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [ACR-2] to Acrosin
  • 特異性The antibody ACR 2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa) in the acrosome of undamaged spermatozoa.
  • アプリケーション適用あり: ICC, WBmore details
  • 種交差性
    交差種: Pig
    非交差種: Cow, Dog, Human
  • 免疫原

    Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC
WB
  • 追加情報ICC: Use at an assay dependent dilution membrane permeabilization (acetone) essential.
    WB: Use at an assay dependent dilution.

    The antibody ACR-2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa) in the acrosome of undamaged spermatozoa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.
    • 配列類似性Belongs to the peptidase S1 family.
      Contains 1 peptidase S1 domain.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Acr antibody
      • ACRO_HUMAN antibody
      • Acrosin heavy chain antibody
      • Acrosin precursor antibody
      • ACRS antibody
      • Preproacrosin antibody
      • Proacrosin antibody
      see all

    Anti-Acrosin antibody [ACR-2] (ab1900) 使用論文

    This product has been referenced in:
    • Moos J  et al. Protein-protein interactions controlling acrosin release and solubilization during the boar sperm acrosome reaction. Biol Reprod 49:408-15 (1993). Read more (PubMed: 8373968) »
    • Moos J  et al. Relationship between molecular conversions of acrosin and the progression of exocytosis in the calcium ionophore-induced acrosome reaction. Biochim Biophys Acta 1176:199-207 (1993). Read more (PubMed: 8471622) »

    See all 4 Publications for this product

    Product Wall

    Thank you for your enquiry. Ab1900 has been tested for application in Immunocytochemistry and Western blotting, and yes, to use it in ICC membrane permeabilization (with acetone) is essential. I'm not aware of an antibody that would bind all sperm,...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"