製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to ACOX2
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rabbit, Orangutan
  • 免疫原

    Synthetic peptide:

    C-QWAQKSPTNTQENP

    , corresponding to internal sequence amino acids 651-664 of Human ACOX2

  • ポジティブ・コントロール
    • Human Liver lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab59516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報
    Peptide ELISA: antibody detection limit dilution 1:4,000.

    WB: Use at a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 77 kDa).

    An additional band of 37kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Oxidizes the CoA esters of the bile acid intermediates di- and tri-hydroxycholestanoic acids.
    • 組織特異性
      Present in all tissues tested: heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Most abundant in heart, liver and kidney.
    • 配列類似性
      Belongs to the acyl-CoA oxidase family.
    • 細胞内局在
      Peroxisome.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 12-alpha-trihydroxy-5-beta-cholestanoyl-CoA 24-hydroxylase antibody
      • 12-alpha-trihydroxy-5-beta-cholestanoyl-CoA oxidase antibody
      • 3 alpha 7 alpha 12 alpha trihydroxy 5 beta cholestanoyl CoA oxidase antibody
      • 3 alpha 7 alpha12 alpha trihydroxy 5 beta cholestanoyl CoA 24 hydroxylase antibody
      • 3-alpha antibody
      • 7-alpha antibody
      • ACOX 2 antibody
      • ACOX2 antibody
      • ACOX2_HUMAN antibody
      • Acyl CoA oxidase 2 antibody
      • Acyl Coenzyme A oxidase 2 antibody
      • Acyl Coenzyme A oxidase 2 branched chain antibody
      • Acyl coenzyme A oxidase 2 peroxisomal antibody
      • BCOX antibody
      • BRCACOX antibody
      • BRCOX antibody
      • Peroxisomal acyl-coenzyme A oxidase 2 antibody
      • Peroxisomal branched chain acyl CoA oxidase antibody
      • THCA CoA oxidase antibody
      • THCA-CoA oxidase antibody
      • THCCox antibody
      • Trihydroxycoprostanoyl CoA oxidase antibody
      • Trihydroxycoprostanoyl-CoA oxidase antibody
      see all

    画像

    • All lanes : Anti-ACOX2 antibody (ab59516) at 0.5 µg/ml

      Lane 1 : Human Liver lysate (35µg protein in RIPA buffer) without blocking with the immunizing peptide
      Lane 2 : Human Liver lysate (35µg protein in RIPA buffer) with blocking with the immunizing peptide

      Developed using the ECL technique

      Predicted band size : 77 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 37 kDa (possible non-specific binding).

    プロトコール

    参考文献

    ab59516 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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