製品の概要

  • 製品名Anti-ACOX1 antibody - Aminoterminal end
    ACOX1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to ACOX1 - Aminoterminal end
  • アプリケーション適用あり: IHC-P, ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide from the N-terminal region or human ACOX1 conjugated to KLH

  • ポジティブ・コントロール
    • Mouse liver lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab59964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/1000.
WB 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).

ターゲット情報

  • 機能Catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. Isoform 1 shows highest activity against medium-chain fatty acyl-CoAs and activity decreases with increasing chain length. Isoform 2 is active against a much broader range of substrates and shows activity towards very long-chain acyl-CoAs. Isoform 2 is twice as active as isoform 1 against 16-hydroxy-palmitoyl-CoA and is 25% more active against 1,16-hexadecanodioyl-CoA.
  • 組織特異性Widely expressed with highest levels of isoform 1 and isoform 2 detected in testis. Isoform 1 is expressed at higher levels than isoform 2 in liver and kidney while isoform 2 levels are higher in brain, lung, muscle, white adipose tissue and testis. Levels are almost equal in heart.
  • パスウェイLipid metabolism; peroxisomal fatty acid beta-oxidation.
  • 関連疾患Adrenoleukodystrophy, pseudoneonatal
  • 配列類似性Belongs to the acyl-CoA oxidase family.
  • 細胞内局在Peroxisome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ACOX antibody
    • ACOX1 antibody
    • ACOX1_HUMAN antibody
    • Acyl CoA oxidase 1 palmitoyl antibody
    • Acyl CoA oxidase straight chain antibody
    • AOX antibody
    • EC 1.3.3.6 antibody
    • PALMCOX antibody
    • Palmitoyl CoA oxidase antibody
    • Palmitoyl-CoA oxidase antibody
    • Peroxisomal acyl coenzyme A oxidase 1 antibody
    • Peroxisomal acyl-coenzyme A oxidase 1 antibody
    • Peroxisomal fatty acyl CoA oxidase antibody
    • SCOX antibody
    • Straight chain acyl CoA oxidase antibody
    • Straight-chain acyl-CoA oxidase antibody
    see all

Anti-ACOX1 antibody - Aminoterminal end 画像

  • Anti-ACOX1 antibody - Aminoterminal end (ab59964) at 1/1000 dilution + Mouse liver lysate

    Predicted band size : 74 kDa
    Observed band size : 74 kDa
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • IHC image of ab59964 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab59964, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-ACOX1 antibody - Aminoterminal end (ab59964) 使用論文

This product has been referenced in:
  • Le TT  et al. Uridine prevents fenofibrate-induced fatty liver. PLoS One 9:e87179 (2014). WB ; Mouse . Read more (PubMed: 24475249) »
  • Wu Y  et al. Differential effects of triclosan on the activation of mouse and human peroxisome proliferator-activated receptor alpha. Toxicol Lett 231:17-28 (2014). Read more (PubMed: 25193434) »

See all 4 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Sample Mouse Tissue lysate - whole (Brain)
Specification Brain
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 May 26 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"