製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ACOT12
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 216-265 (SASRLCWAHP FLKSVDMFKF RGPSTVGDRL VFTAIVNNTF QTCVEVGVRV) of human ACOT12 (NP_570123).

  • ポジティブ・コントロール
    • 721_B cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab83326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titer in peptide based ELISA is 1:12500.

ターゲット情報

  • 機能
    Hydrolyzes acetyl-CoA to acetate and CoA.
  • パスウェイ
    Carbohydrate metabolism; pyruvate metabolism.
  • 配列類似性
    Contains 2 acyl coenzyme A hydrolase domains.
    Contains 1 START domain.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ACO12_HUMAN antibody
    • Acot12 antibody
    • Acyl CoA thioester hydrolase 12 antibody
    • Acyl CoA thioesterase 12 antibody
    • Acyl-CoA thioester hydrolase 12 antibody
    • Acyl-CoA thioesterase 12 antibody
    • Acyl-coenzyme A thioesterase 12 antibody
    • Cach antibody
    • CACH-1 antibody
    • CACH1 antibody
    • Cytoplasmic acetyl CoA hydrolase 1 antibody
    • Cytoplasmic acetyl-CoA hydrolase 1 antibody
    • cytosolic acetyl CoA hydrolase antibody
    • hCACH-1 antibody
    • hCACH1 antibody
    • MGC105114 antibody
    • StAR related lipid transfer (START) domain containing 15 antibody
    • StARD15 antibody
    • START domain containing protein 12 antibody
    • START domain-containing protein 15 antibody
    • THEAL antibody
    see all

画像

  • Anti-ACOT12 antibody (ab83326) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 62 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab83326 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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