Anti-Aconitase 2 抗体 [6D1BE4] (ab110320)
Key features and details
- Mouse monoclonal [6D1BE4] to Aconitase 2
- Suitable for: IP, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-Aconitase 2 antibody [6D1BE4]
Aconitase 2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [6D1BE4] to Aconitase 2 -
由来種
Mouse -
特異性
This antibody is not suitable for Western blot. We recommend ab110321 for Western blotting. -
アプリケーション
適用あり: IP, ICC/IF, Flow Cytmore details
適用なし: WB -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- HDFn cells and HeLa cells; Hela, HepG2, Rat and Mouse liver cell lysates.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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特記事項(精製)
ab110320 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity: >95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
6D1BE4 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab110320の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 1 µg/ml.
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Flow Cyt |
Use a concentration of 1 µg/ml.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
特記事項 |
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IP
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 1 µg/ml. |
Flow Cyt
Use a concentration of 1 µg/ml. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Catalyzes the isomerization of citrate to isocitrate via cis-aconitate. -
パスウェイ
Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2. -
配列類似性
Belongs to the aconitase/IPM isomerase family. -
細胞内局在
Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 50 Human
- Entrez Gene: 11429 Mouse
- Entrez Gene: 79250 Rat
- Omim: 100850 Human
- SwissProt: Q99798 Human
- SwissProt: Q99KI0 Mouse
- SwissProt: Q9ER34 Rat
- Unigene: 643610 Human
see all -
別名
- ACO 2 antibody
- ACO2 antibody
- ACON_HUMAN antibody
see all
画像
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ab110320 at 1µg/ml staining Aconitase 2 in HDFn cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red).
Note: the target protein locates to the mitochondria. -
Detection of ab110321 by colloidal Coomassie blue G staining of Immunprecipitate.
Staining of Aconitase in HeLa, HepG2, Rat liver homogenate (RLH), and Mouse liver homogenate (MLH) lysates immunoprecipitated using ab110320. -
ab110320 at 1µg/ml staining Aconitase 2 in HeLa cells by Flow Cytometry (blue). Isotype control antibody (red).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab110320 は論文での使用が確認できていません。