Anti-ACK1 (phospho Y284) 抗体 (ab74091)
Key features and details
- Rabbit polyclonal to ACK1 (phospho Y284)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-ACK1 (phospho Y284) antibody
ACK1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ACK1 (phospho Y284) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide corresponding to Human ACK1 (phospho Y284).
-
ポジティブ・コントロール
- Human breast carcinoma tissue, A549 cells, and HepG2 cell extracts treated with EGF (200ng/ml, 30mins).
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
ab74091 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab74091の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/500 - 1/1000. Detects a band of approximately >117 kDa (predicted molecular weight: 114 kDa).
|
|
IHC-P |
1/50 - 1/100.
|
|
ICC/IF |
1/500 - 1/1000.
|
特記事項 |
---|
WB
1/500 - 1/1000. Detects a band of approximately >117 kDa (predicted molecular weight: 114 kDa). |
IHC-P
1/50 - 1/100. |
ICC/IF
1/500 - 1/1000. |
ターゲット情報
-
機能
Non-receptor tyrosine kinase that regulates the activity of a number of proteins by tyrosine phosphorylation especially proteins critical for cell survival, cell growth, and proliferation. Activates AKT1 by phosphorylating it on 'Tyr-176' resulting in its activation. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' and promotes its recruitment to the androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR. Participates in clathrin-mediated endocytosis. May be involved both in adult synaptic function and plasticity and in brain development. -
組織特異性
The Tyr-284 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages. It also shows a significant increase in expression in prostate cancers during the progressive stages. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.
Contains 1 SH3 domain.
Contains 1 UBA domain. -
ドメイン
The EBD (EGFR-binding domain) domain is necessary for interaction with EGFR.
The SAM-like domain is necessary for NEDD4-mediated ubiquitination. Promotes membrane localization and dimerization to allow for autophosphorylation.
The UBA domain binds both poly- and mono-ubiquitin. -
翻訳後修飾
Autophosphorylation regulates kinase activity. Phosphorylation on Tyr-518 is required for interaction with SRC.
Ubiquitinated by NEDD4. Its EGF-induced degradation is EGFR activation-dependent and is processed by lysosomes, not proteasomes. -
細胞内局在
Cell membrane. Nucleus. Endosome. Cell junction > adherens junction. Cytoplasmic vesicle membrane. The Tyr-284 phosphorylated form is expressed both in the membrane and nucleus. Co-localizes with EGFR on the endosomes. - Information by UniProt
-
参照データベース
- Entrez Gene: 10188 Human
- Entrez Gene: 51789 Mouse
- Omim: 606994 Human
- SwissProt: Q07912 Human
- SwissProt: O54967 Mouse
- Unigene: 518513 Human
- Unigene: 251115 Mouse
-
別名
- Acetate kinase 1 antibody
- Acetokinase 1 antibody
- ACK 1 antibody
see all
画像
-
All lanes : Anti-ACK1 (phospho Y284) antibody (ab74091) at 1/500 dilution
Lane 1 : HepG2 cell extracts
treated with EGF (200ng/ml, 30mins)
Lane 2 : HepG2 cell extracts
treated with EGF (200ng/ml, 30mins) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 114 kDa
Observed band size: >117 kDa why is the actual band size different from the predicted? -
ab74091 at 1/500 dilution staining ACK1 in A549 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
-
ab74091 at 1/50 dilution staining ACK1 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (6)
ab74091 は 6 報の論文で使用されています。
- Zhang T et al. Discovery of a novel third-generation EGFR inhibitor and identification of a potential combination strategy to overcome resistance. Mol Cancer 19:90 (2020). PubMed: 32404161
- Fritz KR et al. Cdc42 activation by endothelin regulates neural crest cell migration in the cardiac outflow tract. Dev Dyn 248:795-812 (2019). PubMed: 31219639
- Shen R et al. The clinicopathological significance of ubiquitin-conjugating enzyme E2C, leucine-rich repeated-containing G protein-coupled receptor, WW domain-containing oxidoreductase, and vasculogenic mimicry in invasive breast carcinoma. Medicine (Baltimore) 98:e15232 (2019). PubMed: 31008954
- Maxson JE et al. Identification and Characterization of Tyrosine Kinase Nonreceptor 2 Mutations in Leukemia through Integration of Kinase Inhibitor Screening and Genomic Analysis. Cancer Res 76:127-38 (2016). PubMed: 26677978
- Xie B et al. ACK1 promotes hepatocellular carcinoma progression via downregulating WWOX and activating AKT signaling. Int J Oncol 46:2057-66 (2015). PubMed: 25738261
- Strochlic TI et al. Ack kinase regulates CTP synthase filaments during Drosophila oogenesis. EMBO Rep N/A:N/A (2014). PubMed: 25223282