The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 151 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
機能Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Induces apoptotic chromatin condensation after activation by CASP3.
配列類似性Contains 1 SAP domain.
翻訳後修飾Undergoes proteolytic cleavage; the processed form is active, contrary to the uncleaved form.