The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報ICC: Use at an assay dependent dilution.
IHC-P: 1/50 - 1/100. Use streptavidin-biotin system or polymer system, incubate for 30 minutes at room temperature. Antigen retrieval is required, boil tissue in 10 mM citrate, pH 6.0 for 10-15 minutes followed by cooling for 10-15 minutes at RT.
IHC-Fr: 1/50 - 1/100.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
関連性Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin.