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Abcam's Acid Phosphatase Assay Kit (Fluorometric) uses non-fluorescent Methylumbelliferyl phosphate disodium (MUP) as the substrate which has Ex/Em=360/440 nm when dephosphorylated by AP. The kit is an ultra-sensitive, simple, direct and HTS-ready fluorometric assay designed to measure AP activity in serum and other bio-samples.
Visit our FAQs page for tips and troubleshooting.
The detection sensitivity is ~1 µU, more sensitive than colorimetric assays.
The kit is suitable for both research and drug discovery.
|AP Assay buffer||NM||1 x 100ml|
|AP Enzyme (Lyophilised)||Green||1 vial|
|MUP Substrate||Red||1 vial|
|Stop Solution||WM||1 x 25ml|
Our Abpromise guarantee covers the use of ab83370 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
ab83370 has not yet been referenced specifically in any publications.