製品の概要

  • 製品名
    Anti-Acid Phosphatase antibody
    Acid Phosphatase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Acid Phosphatase
  • 特異性
    This antibody reacts with erythrocyte acid phosphatase.
  • アプリケーション
    適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Cat, Pig
  • 免疫原

    A region within synthetic peptide: SENWRVDSAA TSGYEIGNPP DYRGQSCMKR HGIPMSHVAR QITKEDFATF, corresponding to N terminal amino acids 37-86 of Human Acid Phosphatase

  • ポジティブ・コントロール
    • Jurkat cell lysate, human kidney tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab58688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:312500.

WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 機能
    Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Isoform 3 does not possess phosphatase activity.
  • 組織特異性
    T-lymphocytes express only isoform 2.
  • 配列類似性
    Belongs to the low molecular weight phosphotyrosine protein phosphatase family.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Acid phosphatase 1 soluble antibody
    • Acid phosphatase of erythrocyte antibody
    • ACP1 antibody
    • Adipocyte acid phosphatase antibody
    • Cytoplasmic phosphotyrosyl protein phosphatase antibody
    • HAAP antibody
    • LMW-PTP antibody
    • LMW-PTPase antibody
    • Low molecular weight cytosolic acid phosphatase antibody
    • Low molecular weight phosphotyrosine protein phosphatase antibody
    • PAP1 antibody
    • PAP2 antibody
    • phosphatase, acid, of erythrocyte antibody
    • PPAC_HUMAN antibody
    • Protein tyrosine phosphatase antibody
    • PTPase antibody
    • Purple acid phosphatase antibody
    • Red cell acid phosphatase 1 antibody
    • testicular secretory protein Li 37 antibody
    see all

画像

  • Anti-Acid Phosphatase antibody (ab58688) at 1.25 µg/ml + Jurkat cell lysate at 10µg.

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/ 50,000 dilution.

    Predicted band size : 18 kDa
    Observed band size : 18 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Acid Phosphatase with ab58688 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

参考文献

ab58688 has not yet been referenced specifically in any publications.

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